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Literature summary extracted from

  • Cai, M.; He, L.H.; Yu, T.Y.
    Molecular clone and expression of a NAD+-dependent glycerol-3-phosphate dehydrogenase isozyme gene from the halotolerant alga Dunaliella salina (2013), PLoS ONE, 8, e62287.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.8 expressed in Escherichia coli BL21(DE3) cells Dunaliella salina
1.1.1.8 gene GPDH1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) Dunaliella salina

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.8 chloroplast
-
 Dunaliella salina 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.8 76600
-
-
 Dunaliella salina
1.1.1.8 100000
-
-
 Dunaliella salina

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.8 dihydroxyacetone phosphate + NADH + H+ Dunaliella salina
-
 sn-glycerol 3-phosphate + NAD+
-
 r
1.1.1.8 dihydroxyacetone phosphate + NADH + H+ Dunaliella salina 435
-
 sn-glycerol 3-phosphate + NAD+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+ Dunaliella salina
-
 glycerone phosphate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.8 Dunaliella salina Q52ZA0
-
-
1.1.1.8 Dunaliella salina V9MH41
-
-
1.1.1.8 Dunaliella salina 435 Q52ZA0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.8 dihydroxyacetone phosphate + NADH + H+
-
 Dunaliella salina sn-glycerol 3-phosphate + NAD+
-
 r
1.1.1.8 dihydroxyacetone phosphate + NADH + H+
-
 Dunaliella salina 435 sn-glycerol 3-phosphate + NAD+
-
 r
1.1.1.8 sn-glycerol 3-phosphate + NAD+
-
 Dunaliella salina glycerone phosphate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.8 ? x * 100000, recombinant enzyme, SDS-PAGE Dunaliella salina
1.1.1.8 ? x * 76600, about, sequence calculation, x* 100000, recombinant His-tagged enzyme, SDS-PAGE Dunaliella salina
1.1.1.8 ? x * 76600, calculated from amino acid sequence Dunaliella salina

Synonyms

EC Number Synonyms Comment Organism
1.1.1.8 G3PDH
-
 Dunaliella salina
1.1.1.8 NAD+-dependent glycerol-3-phosphate dehydrogenase
-
 Dunaliella salina

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.8 NAD+
-
 Dunaliella salina
1.1.1.8 NADH
-
 Dunaliella salina

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.8 Dunaliella salina calculated from amino acid sequence
-
 6.5

General Information

EC Number General Information Comment Organism
1.1.1.8 metabolism glycerol-3-phosphate dehydrogenase (G3PDH) is a key enzyme in the pathway of glycerol synthesis, which converts dihydroxyacetone phosphate (DHAP) to glycerol-3-phosphate Dunaliella salina
1.1.1.8 physiological function the glycerol-DHAP cycle pathway, which is driven by enzyme G3PDH, is considered as the rate-limiting enzyme to regulate the glycerol level under osmotic shocks Dunaliella salina