Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yadav, R.; Prasad, R.
    Identification and functional characterization of sorbitol-6-phosphate dehydrogenase protein from rice and structural elucidation by in silico approach (2014), Planta, 240, 223-238.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.200 expressed in Escherichia coli BL21 cells Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.200 7.21
-
 D-sorbitol 6-phosphate at pH 8.0 and 25°C Oryza sativa
1.1.1.200 15.9
-
 D-glucose 6-phosphate at pH 8.0 and 25°C Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.200 39000
-
 x * 39000, SDS-PAGE Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.200 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.200 Ni-NTA column chromatography Oryza sativa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.200 0.477
-
 with D-sorbitol 6-phosphate as substrate, at pH 8.0 and 25°C Oryza sativa
1.1.1.200 0.603
-
 with D-glucose 6-phosphate as substrate, at pH 8.0 and 25°C Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.200 D-glucose 6-phosphate + NADPH + H+
-
 Oryza sativa D-sorbitol 6-phosphate + NADP+
-
 r
1.1.1.200 D-sorbitol 6-phosphate + NADP+
-
 Oryza sativa D-glucose 6-phosphate + NADPH + H+
-
 r
1.1.1.200 additional information no activity against mannose 6-phosphate Oryza sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.200 ? x * 39000, SDS-PAGE Oryza sativa

Synonyms

EC Number Synonyms Comment Organism
1.1.1.200 S6PDH
-
 Oryza sativa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.200 NADP+
-
 Oryza sativa
1.1.1.200 NADPH
-
 Oryza sativa

General Information

EC Number General Information Comment Organism
1.1.1.200 physiological function sorbitol-6-phosphate dehydrogenase is a key enzyme for sorbitol synthesis and plays an important role in the alleviation of salinity stress in plants Oryza sativa