Any feedback?
Literature summary extracted from
- Two types of alcohol dehydrogenase from Perilla can form citral and perillaldehyde (2014), Phytochemistry, 104, 12-20.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.183 | DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis | Perilla frutescens |
| 1.1.1.183 | DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis | Perilla frutescens var. hirtella |
| 1.1.1.183 | DNA and amino acid sequence determination, comparison, and analysis, semi-quantitative RT-PCR enzyme expression analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS | Perilla frutescens var. hirtella |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.183 | geraniol + NADP+ | Perilla frutescens | - |
geranial + NADPH + H+ | - |
? |  |
| 1.1.1.183 | geraniol + NADP+ | Perilla frutescens var. hirtella | - |
geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | Perilla frutescens var. hirtella | - |
geranial + NADPH + H+ | - |
r | |
| 1.1.1.183 | geraniol + NADP+ | Perilla frutescens 32 | - |
geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | Perilla frutescens var. hirtella 5031 | - |
geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | Perilla frutescens var. hirtella 87 | - |
geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | Perilla frutescens var. hirtella 87 | - |
geranial + NADPH + H+ | - |
r | |
| 1.1.1.183 | additional information | Perilla frutescens var. hirtella | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products | ? | - |
? | |
| 1.1.1.183 | additional information | Perilla frutescens | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | ? | - |
? | |
| 1.1.1.183 | additional information | Perilla frutescens 32 | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | ? | - |
? | |
| 1.1.1.183 | additional information | Perilla frutescens var. hirtella 87 | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.183 | Perilla frutescens | K9S1J6 | - |
- |
| 1.1.1.183 | Perilla frutescens | K9S1J6 | PA type | - |
| 1.1.1.183 | Perilla frutescens 32 | K9S1J6 | PA type | - |
| 1.1.1.183 | Perilla frutescens var. hirtella | - |
- |
- |
| 1.1.1.183 | Perilla frutescens var. hirtella | K9RZL7 | - |
- |
| 1.1.1.183 | Perilla frutescens var. hirtella | K9RZL7 | C type | - |
| 1.1.1.183 | Perilla frutescens var. hirtella | K9S0X0 | - |
- |
| 1.1.1.183 | Perilla frutescens var. hirtella | K9S0X0 | SF type | - |
| 1.1.1.183 | Perilla frutescens var. hirtella 5031 | K9S0X0 | SF type | - |
| 1.1.1.183 | Perilla frutescens var. hirtella 87 | K9RZL7 | C type | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.183 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS | Perilla frutescens var. hirtella |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.183 | leaf | - |
Perilla frutescens | - |
| 1.1.1.183 | leaf | - |
Perilla frutescens var. hirtella | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.183 | geranial + NADPH + H+ | - |
Perilla frutescens var. hirtella | geraniol + NADP+ | - |
r | |
| 1.1.1.183 | geranial + NADPH + H+ | - |
Perilla frutescens var. hirtella 87 | geraniol + NADP+ | - |
r | |
| 1.1.1.183 | geranial + NADPH + H+ | - |
Perilla frutescens var. hirtella | nerol + NADP+ | PcGeDH can reduce citral to geraniol and nerol when NADPH is added as cofactor, but not when NADP+ is the cofactor. PcGeDH can catalyze either oxidation or reduction. Oil component identification by gas mass spectrometry | r | |
| 1.1.1.183 | geranial + NADPH + H+ | - |
Perilla frutescens var. hirtella 87 | nerol + NADP+ | PcGeDH can reduce citral to geraniol and nerol when NADPH is added as cofactor, but not when NADP+ is the cofactor. PcGeDH can catalyze either oxidation or reduction. Oil component identification by gas mass spectrometry | r | |
| 1.1.1.183 | geraniol + NADP+ | - |
Perilla frutescens | geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | - |
Perilla frutescens var. hirtella | geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | - |
Perilla frutescens var. hirtella | geranial + NADPH + H+ | - |
r | |
| 1.1.1.183 | geraniol + NADP+ | - |
Perilla frutescens 32 | geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | - |
Perilla frutescens var. hirtella 5031 | geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | - |
Perilla frutescens var. hirtella 87 | geranial + NADPH + H+ | - |
? | |
| 1.1.1.183 | geraniol + NADP+ | - |
Perilla frutescens var. hirtella 87 | geranial + NADPH + H+ | - |
r | |
| 1.1.1.183 | additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products | Perilla frutescens var. hirtella | ? | - |
? | |
| 1.1.1.183 | additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | Perilla frutescens | ? | - |
? | |
| 1.1.1.183 | additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | Perilla frutescens var. hirtella | ? | - |
? | |
| 1.1.1.183 | additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | Perilla frutescens 32 | ? | - |
? | |
| 1.1.1.183 | additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products. Oil component identification by gas mass spectrometry | Perilla frutescens var. hirtella 5031 | ? | - |
? | |
| 1.1.1.183 | additional information | the overall reaction from geranyl diphosphate to citral is performed in vitro using geraniol synthase and GeDH from Perilla to form a large proportion of citral and relatively little geraniol as reaction products | Perilla frutescens var. hirtella 87 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.183 | GeDH | - |
Perilla frutescens |
| 1.1.1.183 | GeDH | - |
Perilla frutescens var. hirtella |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.183 | 30 | - |
assay at | Perilla frutescens |
| 1.1.1.183 | 30 | - |
assay at | Perilla frutescens var. hirtella |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.1.183 | 7 | - |
assay at | Perilla frutescens |
| 1.1.1.183 | 7 | - |
assay at | Perilla frutescens var. hirtella |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.183 | NADP+ | - |
Perilla frutescens | |
| 1.1.1.183 | NADP+ | - |
Perilla frutescens var. hirtella | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.183 | evolution | GeDH gene expression in C-, PA-, PK-, and PP-type Perilla | Perilla frutescens |
| 1.1.1.183 | evolution | GeDH gene expression in C-, PA-, PK-, and PP-type Perilla | Perilla frutescens var. hirtella |
| 1.1.1.183 | metabolism | the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil | Perilla frutescens |
| 1.1.1.183 | metabolism | the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil | Perilla frutescens var. hirtella |
| 1.1.1.183 | metabolism | the enzyme geraniol dehydrogenase is involved in the biosynthetic pathway from geranyl diphosphate to citral, the main compound of citral-type perilla essential oil. Geranial is formed from geraniol by enzymatic reaction of Perilla aldo-keto reductase or Perilla GeDH | Perilla frutescens var. hirtella |
| 1.1.1.183 | physiological function | two types of alcohol dehydrogenases, an aldo-keto reductase (AKR) and a geraniol dehydrogenase (GeDH), are thought to participate in the biosynthesis of perilla essential oil components, such as citral and perillaldehyde | Perilla frutescens |
| 1.1.1.183 | physiological function | two types of alcohol dehydrogenases, an aldo-keto reductase (AKR) and a geraniol dehydrogenase (GeDH), are thought to participate in the biosynthesis of perilla essential oil components, such as citral and perillaldehyde | Perilla frutescens var. hirtella |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
