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Literature summary extracted from

  • Terashima, M.; Fujita, Y.; Togashi, Y.; Sakai, K.; De Velasco, M.A.; Tomida, S.; Nishio, K.
    KIAA1199 interacts with glycogen phosphorylase kinase beta-subunit (PHKB) to promote glycogen breakdown and cancer cell survival (2014), Oncotarget, 5, 7040-7050.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 additional information KIAA1199 enhanced the phosphorylation of glycogen phosphorylase brain form Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ca2+ required Homo sapiens
2.7.11.19 Mg2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.19 125000
-
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.19 2 ATP + glycogen phosphorylase b Homo sapiens
-
 2 ADP + glycogen phosphorylase a
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Homo sapiens Q93100
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 gastric cancer cell
-
 Homo sapiens
-
2.7.11.19 Hep-G2 cell
-
 Homo sapiens
-
2.7.11.19 KATO-III cell
-
 Homo sapiens
-
2.7.11.19 SNU-16 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 2 ATP + glycogen phosphorylase b
-
 Homo sapiens 2 ADP + glycogen phosphorylase a
-
 ?
2.7.11.19 2 ATP + glycogen phosphorylase b glycogen phosphorylase brain form, PYGB Homo sapiens 2 ADP + glycogen phosphorylase a
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.19 ? x * 125000, about, beta-subunit Homo sapiens
2.7.11.19 More PHK is an enzyme comprised of four subunits, of which the gamma-subunit possesses enzymatic activity that is tightly regulated by the other three regulatory subunits in response to changes in intracellular Ca2+ concentrations (sensed by the delta-subunit, calmodulin) and cAMP levels that stimulate protein kinase A to phosphorylate the alpha- and beta-subunits (PHKA and PHKB). Upon phosphorylation, these two subunits, which basically inhibit the activity of the gamma-subunit, reduce their function as gamma-inhibitors, resulting in the enzymatic activation of PHK Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.7.11.19 Glycogen phosphorylase kinase
-
 Homo sapiens
2.7.11.19 PhK
-
 Homo sapiens
2.7.11.19 PHKB
-
 Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.19 ATP
-
 Homo sapiens
2.7.11.19 Calmodulin the delta subunit Homo sapiens

General Information

EC Number General Information Comment Organism
2.7.11.19 malfunction KIAA1199 protein silencing by siRNA results in a marked reduction in the phosphorylation of glycogen phosphorylase brain form, PYGB, by PHK Homo sapiens
2.7.11.19 metabolism proposed model of glycogen metabolism through the activation of PHK and PYG pathways in cancer cells with or without KIAA1199 overexpression, overview. PYG plays a central role in the mobilization of carbohydrate reserves in a wide variety of organs and tissues Homo sapiens
2.7.11.19 physiological function glycogen phosphorylase kinase (PHK) is a serine/threonine-specific protein kinase that phosphorylates a serine residue in glycogen phosphorylase (PYG), triggering the activation of this latter enzyme that catalyzes the phosphorolytic cleavage of the alpha-1,4 glycosidic linkages of glycogen, releasing glucose-1-phosphate as the reaction product. The glycogen phosphorylase kinase beta-subunit (PHKB) interacts with the C-terminal region of KIAA1199 protein, that is associated with cancer cell migration or invasion and a poor prognosis and with non-syndromic hearing loss. KIAA1199 protein also interacts with glycogen phosphorylase brain form (PYGB) under serum-free conditions. The interaction promotes glycogen breakdown and cancer cell survival. KIAA1199 binding protein pull down assay, overview Homo sapiens