Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chakravarty, A.K.; Shuman, S.
    The sequential 2',3'-cyclic phosphodiesterase and 3'-phosphate/5'-OH ligation steps of the RtcB RNA splicing pathway are GTP-dependent (2012), Nucleic Acids Res., 40, 8558-8567.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.8 H337A His337 is the site covalent guanylylation of RtcB, mutant is inactive Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.8 Mn2+ absolutely required, assay at 2 mM Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.8 Escherichia coli P46850
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O substrate for ligation is a 20mer RNA strand with 5'-OH and 2',3'-cyclic phosphate ends Escherichia coli (ribonucleotide)n+m + GMP + diphosphate overall reaction ?
6.5.1.8 5'-guanosyl [RNA ligase]-NT-phosphono-L-histidine + (ribonucleotide)n-3'-phosphate
-
 Escherichia coli (ribonucleotide)n-3'-(5'-diphosphoguanosine) + [RNA ligase]-L-histidine RtcB transfers GMP to the RNA 3'-phosphate end ?
6.5.1.8 GTP + [RNA ligase]-L-histidine
-
 Escherichia coli 5'-guanosyl [RNA ligase]-NT-phosphono-L-histidine + diphosphate
-
 ?
6.5.1.8 additional information sealing of a 2',3'-cyclic phosphate end by RtcB requires GTP, is contingent on formation of the RtcB–GMP adduct, and involves a kinetically valid RNA(3')pp(5')G intermediate. RtcB catalyzes the hydrolysis of a 2',3'-cyclic phosphate to a 3'-phosphate at a rate that is at least as fast as the rate of ligation. NTP requirement for ligation is satisfied by GTP and 6-O-methyl guanosine triphosphate and inosine triphosphate. 6-chloropurine ribonucleoside triphosphate, 2-aminopurine ribonucleoside triphosphate and ATP are inactive Escherichia coli ?
-
 ?

General Information

EC Number General Information Comment Organism
6.5.1.8 physiological function RtcB guanylylation precedes the cyclic phosphodiesterase and 3'-phosphate ligase steps of the RNA splicing pathway Escherichia coli