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Literature summary extracted from

  • Ortiz, C.; Moraca, F.; Medeiros, A.; Botta, M.; Hamilton, N.; Comini, M.; Schmidt, T.
    Binding mode and selectivity of steroids towards glucose-6-phosphate dehydrogenase from the pathogen Trypanosoma cruzi (2016), Molecules, 21, 368.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.49 expressed in Escherichia coli BL21(DE3) cells Trypanosoma cruzi

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.49 K83A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
1.1.1.49 K84A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
1.1.1.49 L80G the mutant shows decreased catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi
1.1.1.49 R408A the mutant shows decreased catalytic efficiency compared to the wild type enzyme Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.49 (3alpha)-3,21-dihydroxypregnan-20-one
-
 Trypanosoma cruzi
1.1.1.49 16-bromoepiandrosterone no activity at 0.03 mM Trypanosoma cruzi
1.1.1.49 epiandrosterone uncompetitive inhibitor, 12% residual activity at 0.03 mM Trypanosoma cruzi
1.1.1.49 N-ethyl-N'-[(3alpha)-17-oxoandrostan-3-yl]urea
-
 Trypanosoma cruzi
1.1.1.49 N-[(3alpha)-17-oxoandrostan-3-yl]sulfuric diamide
-
 Trypanosoma cruzi
1.1.1.49 N-[(3alpha)-21-hydroxy-20-oxopregnan-3-yl]sulfuric diamide
-
 Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 0.016
-
 NADP+ wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.017
-
 NADP+ mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.017
-
 NADP+ mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.021
-
 NADP+ mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.074
-
 D-glucose 6-phosphate mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.075
-
 NADP+ mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.077
-
 D-glucose 6-phosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.168
-
 D-glucose 6-phosphate mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.537
-
 D-glucose 6-phosphate mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.618
-
 D-glucose 6-phosphate mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+ Trypanosoma cruzi
-
 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Trypanosoma cruzi Q1WBU6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49 HisTrap column chromatography and Superdex 200 gel filtration Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Trypanosoma cruzi 6-phospho-D-glucono-1,5-lactone + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 G6PDH
-
 Trypanosoma cruzi
1.1.1.49 glucose-6-phosphate dehydrogenase
-
 Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.49 2
-
 D-glucose 6-phosphate mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 2.7
-
 NADP+ mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 2.8
-
 D-glucose 6-phosphate mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 3
-
 NADP+ mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 15
-
 D-glucose 6-phosphate mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 19
-
 NADP+ mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 24.6
-
 D-glucose 6-phosphate mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 52
-
 NADP+ wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 58
-
 NADP+ mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 62
-
 D-glucose 6-phosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NADP+
-
 Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.49 0.0025
-
 epiandrosterone wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.003
-
 epiandrosterone mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.008
-
 epiandrosterone mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.011
-
 epiandrosterone mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 0.031
-
 epiandrosterone mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.1.1.49 0.000015
-
 wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi 16-bromoepiandrosterone
1.1.1.49 0.0015
-
 wild type enzyme, at pH 7.5 and 25° Trypanosoma cruzi N-ethyl-N'-[(3alpha)-17-oxoandrostan-3-yl]urea
1.1.1.49 0.0022
-
 wild type enzyme, at pH 7.5 and 25° Trypanosoma cruzi N-[(3alpha)-17-oxoandrostan-3-yl]sulfuric diamide
1.1.1.49 0.003
-
 wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi epiandrosterone

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.49 5
-
 D-glucose 6-phosphate mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 27
-
 D-glucose 6-phosphate mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 36
-
 NADP+ mutant enzyme L80G, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 40
-
 D-glucose 6-phosphate mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 89
-
 D-glucose 6-phosphate mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 140
-
 NADP+ mutant enzyme K83A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 800
-
 D-glucose 6-phosphate wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 1100
-
 NADP+ mutant enzyme R408A, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 3200
-
 NADP+ wild type enzyme, at pH 7.5 and 25°C Trypanosoma cruzi
1.1.1.49 3400
-
 NADP+ mutant enzyme K84A, at pH 7.5 and 25°C Trypanosoma cruzi