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Literature summary extracted from

  • Loprasert, S.
    Cloning of toluene 4-monooxygenase genes and application of two-phase system to the production of the anticancer agent, indirubin (2015), Mol. Biotechnol., 57, 720-726.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.13.236 synthesis development of a two-phase (dioctyl phthalate/aqueous medium) culture system to obtain maximal indirubin from indole. A 50% (v/v) dioctyl phthalate two-phase system using tryptophan medium containing 3 mM cysteine, 5 mM indole, and 1 mM isatin yields 102.4 mg/l of indirubin with no conversion of indole to indigo Pseudomonas sp. M4

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.236 expression in Escherichia coli Pseudomonas sp. M4

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.236 Pseudomonas sp. M4 A0A0D3QM77 and A0A0D3QME2 and A0A0D3QLU4 and A0A0D3QM47 and A0A0D3QMJ7 and A0A0D3QM80 A0A0D3QM77 i.e. subunit TmoA, A0A0D3QME2 i.e. subunit TmoB, A0A0D3QLU4 i.e. subunit TmoC, A0A0D3QM47 i.e. subunit TmD, A0A0D3QMJ7 i.e. subunit TmoE, A0A0D3QM80 i.e. subunit TmoF
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.236 2 indole + 3 NADH + 3 H+ + 3 O2
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 Pseudomonas sp. M4 indirubin + 3 NAD+ + 3 H2O reaction via C-3 oxidation to indoxyl, oxidation to isatin, and recombinantion of isatin and indoxyl to indirubin ?