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Literature summary extracted from

  • Chen, W.; Cheng, X.; Zhou, Z.; Liu, J.; Wang, H.
    Molecular cloning and characterization of a tropinone reductase from Dendrobium nobile Lindl (2013), Mol. Biol. Rep., 40, 1145-1154.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.206 gene DnTR1, sequence comparisons and unrooted phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), realtime PCR enzyme expression analysis Dendrobium nobile

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.206 additional information
-
 additional information Michaelis-Menten kinetics Dendrobium nobile
1.1.1.206 0.167
-
 3-quinuclidinone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile
1.1.1.206 5.61
-
 tropinone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile
1.1.1.206 8.71
-
 4-methylcyclohexanone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.206 28000
-
-
 Dendrobium nobile
1.1.1.206 60000
-
 gel filtration, recombinant enzyme Dendrobium nobile

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.206 tropinone + NADPH + H+ Dendrobium nobile
-
 tropine + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.206 Dendrobium nobile H9BQR7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.206 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration, followed by ultrafiltration Dendrobium nobile

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.206 leaf
-
 Dendrobium nobile
-
1.1.1.206 additional information DnTR1 may be ubiquitously expressed in all tissues, expression profile of DnTR1 Dendrobium nobile
-
1.1.1.206 root
-
 Dendrobium nobile
-
1.1.1.206 stem
-
 Dendrobium nobile
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.206 3-quinuclidinone + NADPH + H+
-
 Dendrobium nobile 1-azabicyclo[2.2.2]octan-3-ol + NADP+
-
 r
1.1.1.206 4-methylcyclohexanone + NADPH + H+
-
 Dendrobium nobile 4-methylcyclohexanol + NADP+
-
 r
1.1.1.206 tropinone + NADPH + H+
-
 Dendrobium nobile tropine + NADP+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.206 homodimer 2 * 28000, recombinant enzyme, SDS-PAGE Dendrobium nobile
1.1.1.206 More three-dimensional structure modelling of DnTR1, overview Dendrobium nobile

Synonyms

EC Number Synonyms Comment Organism
1.1.1.206 DnTR1
-
 Dendrobium nobile

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.206 25
-
 assay at Dendrobium nobile

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.206 8
-
 assay at Dendrobium nobile

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.206 NADP+
-
 Dendrobium nobile
1.1.1.206 NADPH
-
 Dendrobium nobile

Expression

EC Number Organism Comment Expression
1.1.1.206 Dendrobium nobile salicylic acid and sodium nitroprusside treatments do not affect the enzyme expression additional information
1.1.1.206 Dendrobium nobile the expression of DnTR1 mRNA is enhanced 9.5times by treatment of methyl jasmonate after 24 h, expression profile of DnTR1 up

General Information

EC Number General Information Comment Organism
1.1.1.206 evolution the enzyme belongs to the short chain dehydrogenase/reductase (SDR) superfamily that is composed of a group of NAD(P)H-dependent oxidoreductases that typically consist of 250-350 amino acids. Tropinone reductases (TRs) are a group of the SDR superfamily, that use the NADPH as coenzyme to reduce tropinone DnTR1 also contains the sequence pattern G-X3-G-X-G in the cofactor binding motif and active site motif (S-N-K) Dendrobium nobile
1.1.1.206 metabolism tropinone reductases form a branch point in the pathway leading to tropine (TRI) and to pseudotropine (TRII) during the tropane alkaloid biosynthesis, overview. Enzyme TRI is involved in the formation of tropine, the preproduct of hyoscyamine, whereas enzyme TRII, EC 1.1.1.236, is responsible for the generation of pseudotropine Dendrobium nobile
1.1.1.206 additional information three-dimensional structure modelling of DnTR1, overview. DnTR1 also contains the sequence pattern G-X3-G-X-G in the cofactor binding motif and active site motif (S-N-K). The positive and negative charges on the binding pockets surface of DsTRI are due to His112. The three catalytic residues are Ser158, Tyr171, and Lys175 in DsTRI Dendrobium nobile
1.1.1.206 physiological function DnTR1 regulation may be involved in a jasmonate-dependent pathway Dendrobium nobile

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.206 0.00094
-
 tropinone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile
1.1.1.206 0.0032
-
 4-methylcyclohexanone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile
1.1.1.206 22.18
-
 3-quinuclidinone pH 8.0, 25°C, recombinant His-tagged enzyme Dendrobium nobile