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Literature summary extracted from

  • Li, K.; Mao, X.; Liu, L.; Lin, J.; Sun, M.; Wei, D.; Yang, S.
    Overexpression of membrane-bound gluconate-2-dehydrogenase to enhance the production of 2-keto-D-gluconic acid by Gluconobacter oxydans (2016), Microb. Cell Fact., 15, 121.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.1.1.215 synthesis ga2dh-overexpressing strain Gluconobacter oxydans_tufB_ga2dh is a useful species for use in 2-dehydro-D-gluconate production Gluconobacter oxydans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.215 gene ga2dh, recombinant overexpression of the enzyme in Gluconobacter oxydans strain DSM2003 under the control of three promoters, PtufB, Pga2dh or Pghp0169, respectively. Recombinant strain Gluconobacter oxydans_tufB_ga2dh shows a similar growth rate to that of the control strain and displays the highest specific productivity of 2-dehydro-D-gluconate from D-gluconate, which is increased nearly twofold compared with that of the control strain during batch biotransformation, method optimization Gluconobacter oxydans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.215 additional information development of an efficient bacterial strain of Gluconobacter oxydans_tufB_ga2dh for the production of 2-dehydro-D-gluconate by overexpressing the ga2dh gene in Gluconobacter oxydans. Supply of sufficient oxygen enhances the positive effect of gene overexpression on 2-dehydro-D-gluconate production. Gluconobacter oxydans_tufB_ga2dh is a competitive species for use in 2-dehydro-D-gluconate production. Transgenic strains, evaluation and optimization, detailed overview Gluconobacter oxydans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.215 membrane membrane-bound Gluconobacter oxydans 16020
-
1.1.99.3 membrane
-
 Gluconobacter oxydans 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.215 D-gluconate + NADP+ Gluconobacter oxydans
-
 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.215 D-gluconate + NADP+ Gluconobacter oxydans 621H
-
 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.215 additional information Gluconobacter oxydans for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate ?
-
 ?
1.1.1.215 additional information Gluconobacter oxydans 621H for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Gluconobacter oxydans Q5FRK5
-
-
1.1.1.215 Gluconobacter oxydans 621H Q5FRK5
-
-
1.1.99.3 Gluconobacter oxydans
-
-
-
1.1.99.3 Gluconobacter oxydans DSM 2003
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 D-gluconate + NADP+
-
 Gluconobacter oxydans 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.215 D-gluconate + NADP+
-
 Gluconobacter oxydans 621H 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.215 additional information for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate Gluconobacter oxydans ?
-
 ?
1.1.1.215 additional information for large-scale production of 2-dehydro-D-gluconate, the cells require for formation of 2-dehydro-D-gluconate from D-gluconate oxygen as the final acceptor of electrons formed during the oxidation ofD -gluconate Gluconobacter oxydans 621H ?
-
 ?
1.1.99.3 D-gluconate + acceptor
-
 Gluconobacter oxydans 2-dehydro-D-gluconate + reduced acceptor
-
 ?
1.1.99.3 D-gluconate + acceptor
-
 Gluconobacter oxydans DSM 2003 2-dehydro-D-gluconate + reduced acceptor
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.215 GA2DH
-
 Gluconobacter oxydans
1.1.1.215 gluconate-2-dehydrogenase
-
 Gluconobacter oxydans
1.1.1.215 GOX1230
-
 Gluconobacter oxydans
1.1.99.3 GA2DH
-
 Gluconobacter oxydans
1.1.99.3 gluconate-2-dehydrogenase
-
 Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 NADP+
-
 Gluconobacter oxydans