Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ho, E.; Kahng, H.; Oh, K.
    Characterization of nitroreductase purified from TNT-degrading bacterium, Pseudomonas sp. HK-6 (2004), Korean J. Microbiol. Biotechnol., 32, 230-237.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.B1 2-mercaptoethanol
-
 Pseudomonas sp.
1.7.1.B1 Ag+ about 70% inhibition Pseudomonas sp.
1.7.1.B1 Cu2+ about 70% inhibition Pseudomonas sp.
1.7.1.B1 Hg2+ about 70% inhibition Pseudomonas sp.
1.7.1.B1 additional information not inhibitory: Fe2+ Pseudomonas sp.
1.7.1.16 2-mercaptoethanol
-
 Pseudomonas sp. HK-6
1.7.1.16 Ag+ about 70% inhibition Pseudomonas sp. HK-6
1.7.1.16 Cu2+ about 70% inhibition Pseudomonas sp. HK-6
1.7.1.16 Hg2+ about 70% inhibition Pseudomonas sp. HK-6
1.7.1.16 additional information not inhibitory: Fe2+ Pseudomonas sp. HK-6

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.B1 27000
-
 x * 27000, SDS-PAGE Pseudomonas sp.
1.7.1.16 27000
-
 x * 27000, SDS-PAGE Pseudomonas sp. HK-6

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.B1 Pseudomonas sp.
-
-
-
1.7.1.16 Pseudomonas sp. HK-6
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.B1
-
 Pseudomonas sp.
1.7.1.16
-
 Pseudomonas sp. HK-6

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.B1 5.01
-
 pH 7.0, 30°C Pseudomonas sp.
1.7.1.16 5.01
-
 pH 7.0, 30°C Pseudomonas sp. HK-6

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.B1 2,4,6-trinitrotoluene + 2 NADPH + 2 H+
-
 Pseudomonas sp. N-hydroxy-2-methyl-3,5-dinitroaniline + 2 NADP+ + H2O
-
 ?
1.7.1.B1 additional information enzyme additionally catalyzes reduction of nitrobenzene, reaction of EC 1.7.1.16 Pseudomonas sp. ?
-
 ?
1.7.1.16 additional information enzyme additionally catalyzes reduction of 2,4,6-trinitrotoluene Pseudomonas sp. HK-6 ?
-
 ?
1.7.1.16 nitrobenzene + 2 NADPH + 2 H+
-
 Pseudomonas sp. HK-6 N-phenylhydroxylamine + 2 NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.7.1.B1 ? x * 27000, SDS-PAGE Pseudomonas sp.
1.7.1.16 ? x * 27000, SDS-PAGE Pseudomonas sp. HK-6

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.B1 30
-
-
 Pseudomonas sp.
1.7.1.16 30
-
-
 Pseudomonas sp. HK-6

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.B1 7
-
-
 Pseudomonas sp.
1.7.1.16 7
-
-
 Pseudomonas sp. HK-6