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Literature summary extracted from

  • Qi, X.; Zhu, J.; Luo, Y.; Lin, J.; Wang, X.; Ju, Z.; Chen, F.; Zhu, X.; Chen, H.; Sun, W.; Wang, L.
    Overexpression and characterization of the gene encoding 1,3-propanediol oxidoreductase of Citrobacter freundii (2014), Key Eng. Mater., 636, 121-124.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.202 gene dhaT, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.202 additional information
-
 additional information Michaelis-Menten kinetics Citrobacter freundii
1.1.1.202 8.9
-
 3-hydroxypropanal pH and temperature not specified in the publication Citrobacter freundii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.202 43000
-
-
 Citrobacter freundii
1.1.1.202 440000
-
 recombinant His6-tagged enzyme, gel filtration Citrobacter freundii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.202 3-hydroxypropanal + NADH + H+ Citrobacter freundii
-
 propane-1,3-diol + NAD+
-
 r
1.1.1.202 propane-1,3-diol + NAD+ Citrobacter freundii
-
 3-hydroxypropanal + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.202 Citrobacter freundii P45513
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.202 recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Citrobacter freundii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.202 3-hydroxypropanal + NADH + H+
-
 Citrobacter freundii propane-1,3-diol + NAD+
-
 r
1.1.1.202 propane-1,3-diol + NAD+
-
 Citrobacter freundii 3-hydroxypropanal + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.202 homodecamer 10 * 42927, sequence calculation, 10 * 43000, recombinant His6-tagged enzyme, SDS-PAGE Citrobacter freundii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.202 1,3-propanediol oxidoreductase
-
 Citrobacter freundii
1.1.1.202 DhaT
-
 Citrobacter freundii
1.1.1.202 PDOR
-
 Citrobacter freundii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.202 additional information PDOR requires NAD(H) as a cofactor, but the highly conserved NAD(H) binding fingerprint pattern G-X-G-X-X-G is not present in the amino acid sequence Citrobacter freundii
1.1.1.202 NAD+
-
 Citrobacter freundii
1.1.1.202 NADH
-
 Citrobacter freundii

General Information

EC Number General Information Comment Organism
1.1.1.202 evolution enzyme PDOR shows high similarity with member of the family of type III alcohol dehydrogenases. PDOR requires NAD(H) as a cofactor, but the highly conserved NAD(H) binding fingerprint pattern G-X-G-X-X-G is not present in the amino acid sequence. This is also characteristic of most type III alcohol dehydrogenases Citrobacter freundii
1.1.1.202 metabolism 1,3-propanediol oxidoreductase (PDOR) is the rate-limiting enzyme in 1,3-propandiol synthesis biological pathway. 3-Hydroxypropanal is an intermediary metabolite in the 1,3-propanediol synthesis pathway. It is also an inhibitor to the activity of glycerol dehydratase (GDHt) and PDOR. PDOR is the key rate-limiting enzyme of the 3-HPA transformation and 1,3-PD formation when high concentration of glycerol is used in fermentation Citrobacter freundii