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Literature summary extracted from

  • Li, L.; Wu, X.; Yue, H.Y.; Zhu, Y.C.; Xu, J.
    Myosin light chain kinase facilitates endocytosis of synaptic vesicles at hippocampal boutons (2016), J. Neurochem., 138, 60-73.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 Calmodulin dependent on Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.18 additional information knockdown of endogenous MLCK by two shRNA sequences: shMLCK1 is 5-GCTAGATTTGACTGCAAGATT-3' and shMLCK 2 is 5'-ACTGTCCTCTATGGCAATGAT-3' Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 ML-7 chemical inhibition of myosin light chain kinase (MLCK) slows down endocytosis Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.18 synaptic vesicle
-
 Rattus norvegicus 8021
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ dependent on, activates Rattus norvegicus
2.7.11.18 Mg2+ required Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] Rattus norvegicus myyosin II ADP + [myosin light chain] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Rattus norvegicus P18666
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 hippocampus hippocampal boutons Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] myyosin II Rattus norvegicus ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain] myosin II Rattus norvegicus ADP + [myosin light chain] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 MLCK
-
 Rattus norvegicus
2.7.11.18 Myl12b
-
 Rattus norvegicus
2.7.11.18 myosin light chain kinase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 22 24 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.18 7.4
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
2.7.11.18 malfunction induced endocytosis is slowed by acute inhibition of MLCK and by downregulation of MLCK via RNA interference while the total amount of vesicle exocytosis and somatic Ca2+channel current does not change with MLCK down-regulation. Downregulation of MLCK prevents depolarization-induced phosphorylation of myosin light chain, an effect shared by blockers of Ca2+ channels and calmodulin. Downregulation of MLCK does not decrease the amount of exocytosis Rattus norvegicus
2.7.11.18 physiological function myosin light chain kinase (MLCK) facilitates endocytosis of synaptic vesicles at hippocampal boutons through activity-dependent phosphorylation of myosin downstream of Ca2+/calmodulin. MLCK is an important activity-dependent regulator of vesicle recycling in hippocampal neurons, which are critical for learning and memory. MLCK positively regulates vesicle endocytosis at hippocampal boutons. MLCK regulates endocytosis by functioning between Ca2+/calmodulin and myosin Rattus norvegicus