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Literature summary extracted from

  • Hyun, J.; Abigail, M.; Choo, J.W.; Ryu, J.; Kim, H.K.
    Effects of N-/C-terminal extra tags on the optimal reaction conditions, activity, and quaternary structure of Bacillus thuringiensis glucose 1-dehydrogenase (2016), J. Microbiol. Biotechnol., 26, 1708-1716.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.47 expressed in Escherichia coli BL21(DE3) cells Bacillus thuringiensis serovar kurstaki

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.47 0.03
-
 NADP+ at pH 9.0 and 55°C Bacillus thuringiensis serovar kurstaki
1.1.1.47 0.17
-
 NAD+ at pH 9.0 and 55°C Bacillus thuringiensis serovar kurstaki
1.1.1.47 0.55
-
 D-glucose at pH 9.0 and 55°C Bacillus thuringiensis serovar kurstaki

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.47 56400
-
-
 Bacillus thuringiensis serovar kurstaki

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.47 D-glucose + NAD+ Bacillus thuringiensis serovar kurstaki
-
 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+ Bacillus thuringiensis serovar kurstaki KCCM 11429
-
 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NADP+ Bacillus thuringiensis serovar kurstaki
-
 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-glucose + NADP+ Bacillus thuringiensis serovar kurstaki KCCM 11429
-
 D-glucono-1,5-lactone + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.47 Bacillus thuringiensis serovar kurstaki
-
-
-
1.1.1.47 Bacillus thuringiensis serovar kurstaki KCCM 11429
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.47 D-fructose + NADP+ 0.03% activity compared to D-glucose Bacillus thuringiensis serovar kurstaki ?
-
 ?
1.1.1.47 D-galactose + NADP+ 1.09% activity compared to D-glucose Bacillus thuringiensis serovar kurstaki ?
-
 ?
1.1.1.47 D-glucose + NAD+
-
 Bacillus thuringiensis serovar kurstaki D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+ 100% activity Bacillus thuringiensis serovar kurstaki D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+
-
 Bacillus thuringiensis serovar kurstaki KCCM 11429 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NAD+ 100% activity Bacillus thuringiensis serovar kurstaki KCCM 11429 D-glucono-1,5-lactone + NADH + H+
-
 ?
1.1.1.47 D-glucose + NADP+
-
 Bacillus thuringiensis serovar kurstaki D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-glucose + NADP+ 100% activity Bacillus thuringiensis serovar kurstaki D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-glucose + NADP+
-
 Bacillus thuringiensis serovar kurstaki KCCM 11429 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-glucose + NADP+ 100% activity Bacillus thuringiensis serovar kurstaki KCCM 11429 D-glucono-1,5-lactone + NADPH + H+
-
 ?
1.1.1.47 D-lactose + NADP+ 3.0% activity compared to D-glucose Bacillus thuringiensis serovar kurstaki ?
-
 ?
1.1.1.47 D-maltose + NADP+ 1.03% activity compared to D-glucose Bacillus thuringiensis serovar kurstaki ?
-
 ?
1.1.1.47 D-mannose + NADP+ 11.53% activity compared to D-glucose Bacillus thuringiensis serovar kurstaki ?
-
 ?
1.1.1.47 D-xylose + NADP+ 12.89% activity compared to D-glucose Bacillus thuringiensis serovar kurstaki ?
-
 ?
1.1.1.47 additional information no activity with sucrose Bacillus thuringiensis serovar kurstaki ?
-
 ?
1.1.1.47 additional information no activity with sucrose Bacillus thuringiensis serovar kurstaki KCCM 11429 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.47 homodimer 2 * 28048, calculated from amino acid sequence Bacillus thuringiensis serovar kurstaki

Synonyms

EC Number Synonyms Comment Organism
1.1.1.47 GDH
-
 Bacillus thuringiensis serovar kurstaki
1.1.1.47 GDHB
-
 Bacillus thuringiensis serovar kurstaki
1.1.1.47 glucose 1-dehydrogenase B
-
 Bacillus thuringiensis serovar kurstaki

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.47 55
-
-
 Bacillus thuringiensis serovar kurstaki

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.47 45 65 more than 85% activity between 45 and 65°C Bacillus thuringiensis serovar kurstaki

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.47 30 65 after 30 min at 30-65°C, the enzyme maintains high activity of more than 85% Bacillus thuringiensis serovar kurstaki

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.47 9
-
-
 Bacillus thuringiensis serovar kurstaki

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.47 7 10 more than 60% activity between pH 7.0 and 10.0 Bacillus thuringiensis serovar kurstaki

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.47 3 11 after 30 min at pH 3.0-11.0, the enzyme maintains activity of more than 70% Bacillus thuringiensis serovar kurstaki

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.47 NAD+
-
 Bacillus thuringiensis serovar kurstaki
1.1.1.47 NADP+
-
 Bacillus thuringiensis serovar kurstaki