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Literature summary extracted from

  • Zhang, L.; Xu, Q.; Peng, X.; Xu, B.; Wu, Y.; Yang, Y.; Sun, S.; Hu, K.; Shen, Y.
    Cloning, expression and characterization of glycerol dehydrogenase involved in 2,3-butanediol formation in Serratia marcescens H30 (2014), J. Ind. Microbiol. Biotechnol., 41, 1319-1327.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.4 gene gldA, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) Serratia marcescens
1.1.1.6 gene gldA, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) Serratia marcescens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.4 Ca2+ inhibits (3S/3R)-acetoin reduction Serratia marcescens
1.1.1.4 Fe2+ strongly inhibits (3S/3R)-acetoin reduction by over 85% Serratia marcescens
1.1.1.4 Fe3+ strongly inhibits (3S/3R)-acetoin reduction by over 85% Serratia marcescens
1.1.1.4 Zn2+ inhibits (3S/3R)-acetoin reduction Serratia marcescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.4 Fe2+ GDH activity is significantly enhanced to 784% for meso-2,3-butanediol oxidation in the presence of Fe2+ Serratia marcescens
1.1.1.4 Mg2+ enhances (3S/3R)-acetoin reduction Serratia marcescens
1.1.1.4 Mn2+ GDH activity is significantly enhanced for (3S/3R)-acetoin reduction in the presence of Mn2+ Serratia marcescens
1.1.1.4 Na+ enhances (3S/3R)-acetoin reduction Serratia marcescens
1.1.1.4 NH4+ enhances (3S/3R)-acetoin reduction Serratia marcescens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.4 39000
-
 x * 39000, recombinant enzyme, SDS-PAGE Serratia marcescens
1.1.1.6 39000
-
 x * 39000, recombinant enzyme, SDS-PAGE Serratia marcescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.6 glycerol + NAD+ Serratia marcescens
-
 glycerone + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.4 Serratia marcescens
-
-
-
1.1.1.4 Serratia marcescens H30
-
-
-
1.1.1.6 Serratia marcescens
-
-
-
1.1.1.6 Serratia marcescens H30
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.4 10.48
-
 crude enzyme, with substrate (3S/3R)-acetoin and NADH at room temperature, pH 8.0, 22°C Serratia marcescens
1.1.1.4 20.75
-
 purified enzyme, with substrate (3S/3R)-acetoin and NADH at room temperature, pH 8.0, 22°C Serratia marcescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.4 (3R,3S)-acetoin + NADH the enzyme shows lower Km value and higher catalytic efficiency for (3S/3R)-acetoin in comparison to those for (2R,3R)-2,3-butanediol and meso-2,3-butanediol, the reduction reaction is preferred, low activity with (2R,3R)-2,3-butanediol Serratia marcescens (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+
-
 r
1.1.1.4 (3R,3S)-acetoin + NADH the enzyme shows lower Km value and higher catalytic efficiency for (3S/3R)-acetoin in comparison to those for (2R,3R)-2,3-butanediol and meso-2,3-butanediol, the reduction reaction is preferred, low activity with (2R,3R)-2,3-butanediol Serratia marcescens H30 (2R,3R)-2,3-butanediol + meso-2,3-butanediol + NAD+
-
 r
1.1.1.4 1,4-butanediol + NAD+ low activity Serratia marcescens ?
-
 r
1.1.1.4 1,4-butanediol + NAD+ low activity Serratia marcescens H30 ?
-
 r
1.1.1.4 diacetyl + NADH + H+ diacetyl can be converted into 2,3-butanediol via acetoin by the enzyme Serratia marcescens acetoin + NAD+
-
 ?
1.1.1.4 diacetyl + NADH + H+ diacetyl can be converted into 2,3-butanediol via acetoin by the enzyme Serratia marcescens H30 acetoin + NAD+
-
 ?
1.1.1.4 isopropanol + NAD+ low activity Serratia marcescens ?
-
 r
1.1.1.4 isopropanol + NAD+ low activity Serratia marcescens H30 ?
-
 r
1.1.1.4 additional information the purified enzyme glycerol dehydrogenase, GDH EC 1.1.1.6, also catalyzes the interconversion of (3S)-acetoin/meso-2,3-butanediol and (3R)-acetoin/(2R,3R)-2,3-butanediol. (2S,3S)-2,3-Butanediol is not a substrate for the GDH at all. Also no activity with 1-propanol, 1-butanol, isopentanol, ethylene glycol, ethanol, and (3S/3R)-acetoin in the oxidation reaction, and poor activity with formaldehyde in the reduction reaction Serratia marcescens ?
-
 ?
1.1.1.4 additional information the purified enzyme glycerol dehydrogenase, GDH EC 1.1.1.6, also catalyzes the interconversion of (3S)-acetoin/meso-2,3-butanediol and (3R)-acetoin/(2R,3R)-2,3-butanediol. (2S,3S)-2,3-Butanediol is not a substrate for the GDH at all. Also no activity with 1-propanol, 1-butanol, isopentanol, ethylene glycol, ethanol, and (3S/3R)-acetoin in the oxidation reaction, and poor activity with formaldehyde in the reduction reaction Serratia marcescens H30 ?
-
 ?
1.1.1.6 glycerol + NAD+
-
 Serratia marcescens glycerone + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.4 ? x * 39000, recombinant enzyme, SDS-PAGE Serratia marcescens
1.1.1.6 ? x * 39000, recombinant enzyme, SDS-PAGE Serratia marcescens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.4 2,3-butanediol dehydrogenase
-
 Serratia marcescens
1.1.1.4 acetoin/diacetyl reductase
-
 Serratia marcescens
1.1.1.4 BDH
-
 Serratia marcescens
1.1.1.4 More cf. EC 1.1.1.6, glycerol dehydrogenase Serratia marcescens
1.1.1.6 GDH
-
 Serratia marcescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.4 60
-
-
 Serratia marcescens
1.1.1.6 60
-
-
 Serratia marcescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.4 20 70 temperature profile, overview Serratia marcescens
1.1.1.6 20 70 temperature profile, overview Serratia marcescens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.4 70
-
 over 60% of maximal activity is retained Serratia marcescens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.4 7
-
 diacetyl reduction Serratia marcescens
1.1.1.4 8
-
 reduction of (3S/3R)-acetoin and oxidations of meso-2,3-butanediol and glycerol Serratia marcescens
1.1.1.6 8
-
-
 Serratia marcescens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.4 5 9 temperature profile, overview Serratia marcescens
1.1.1.6 7 10 temperature profile, overview Serratia marcescens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.4 NAD+
-
 Serratia marcescens
1.1.1.4 NADH
-
 Serratia marcescens
1.1.1.6 NAD+
-
 Serratia marcescens
1.1.1.6 NADH
-
 Serratia marcescens

General Information

EC Number General Information Comment Organism
1.1.1.4 evolution the Serratia marcescens enzyme belongs to the type III Fe-ADH superfamily, three consecutive glycine residues belong to a 14-amino acid residue motif (GDK motif) as the coenzyme NAD(H) binding site, and three conserved histidine residues belong to a 16-residue segment that is homologous to the 15-residue stretch as the binding site of metal Serratia marcescens
1.1.1.6 evolution the Serratia marcescens enzyme belongs to the type III Fe-ADH superfamily, three consecutive glycine residues belong to a 14-amino acid residue motif (GDK motif) as the coenzyme NAD(H) binding site, and three conserved histidine residues belong to a 16-residue segment that is homologous to the 15-residue stretch as the binding site of metal Serratia marcescens