Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bontpart, T.; Marlin, T.; Vialet, S.; Guiraud, J.L.; Pinasseau, L.; Meudec, E.; Sommerer, N.; Cheynier, V.; Terrier, N.
    Two shikimate dehydrogenases, VvSDH3 and VvSDH4, are involved in gallic acid biosynthesis in grapevine (2016), J. Exp. Bot., 67, 3537-3550.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.25 isozyme VvSDH1, sequence comparison and phylogenetic analysis and tree, recombinant expression Vitis vinifera
1.1.1.25 isozyme VvSDH2, sequence comparison and phylogenetic analysis and tree, recombinant expression Vitis vinifera
1.1.1.25 isozyme VvSDH3, sequence comparison and phylogenetic analysis and tree, recombinant expression Vitis vinifera
1.1.1.25 isozyme VvSDH4, sequence comparison and phylogenetic analysis and tree, recombinant expression Vitis vinifera

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.25 0.023
-
 NADP+ pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 0.027
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 0.038
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 0.046
-
 3-dehydroshikimate pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 0.0538
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH4 Vitis vinifera
1.1.1.25 0.07
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 0.114
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 0.1468
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH4 Vitis vinifera
1.1.1.25 0.155
-
 shikimate pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 0.157
-
 3-dehydroshikimate pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 0.217
-
 NADP+ pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 0.422
-
 shikimate pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 186
-
 3-dehydroshikimate pH 7.0, 30°C, recombinant isozyme VvSDH4 Vitis vinifera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.25 chloroplast isozyme VvSDH3 contains a putative chloroplastic transit peptide Vitis vinifera 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.25 shikimate + NADP+ Vitis vinifera
-
 3-dehydroshikimate + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.25 Vitis vinifera A0A168S2B8
-
-
1.1.1.25 Vitis vinifera A0A168S2G5
-
-
1.1.1.25 Vitis vinifera A0A168S2H6
-
-
1.1.1.25 Vitis vinifera D7TUX1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.25 berry
-
 Vitis vinifera
-
1.1.1.25 fruit VvSDH isozymes expression is analysed in the main berry tissues (skin, pulp, and seeds) at three development stages (green stage, veraison, and maturity) to determine their spatial and temporal expression patterns, overview Vitis vinifera
-
1.1.1.25 pericarp high expression is observed for isozyme VvSDH1 before veraison but its maximum expression level is reached after veraison Vitis vinifera
-
1.1.1.25 pericarp the VvSDH3 expression level rises during the green stage. At veraison, its expression drops and stays very low throughout the ripening stage Vitis vinifera
-
1.1.1.25 pericarp VvSDH2 expression is relatively stable during grape development, except for a peak of expression before veraison Vitis vinifera
-
1.1.1.25 pericarp VvSDH4 expression is relatively stable during grape development, except for a peak of expression before veraison Vitis vinifera
-
1.1.1.25 seed
-
 Vitis vinifera
-
1.1.1.25 seed immature, highest expression level of VvSDH3 Vitis vinifera
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.25 additional information isozyme VvSDH1 also produces gallate from shikimate with NADP+ Vitis vinifera ?
-
 ?
1.1.1.25 additional information isozyme VvSDH2 does not produce gallate from shikimate Vitis vinifera ?
-
 ?
1.1.1.25 additional information isozyme VvSDH3 also produces gallate from shikimate with NADP+ with low activity Vitis vinifera ?
-
 ?
1.1.1.25 additional information isozyme VvSDH4 also produces gallate from shikimate with NADP+ with low activity Vitis vinifera ?
-
 ?
1.1.1.25 shikimate + NADP+
-
 Vitis vinifera 3-dehydroshikimate + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.25 SDH
-
 Vitis vinifera
1.1.1.25 VvSDH1
-
 Vitis vinifera
1.1.1.25 VvSDH2
-
 Vitis vinifera
1.1.1.25 VvSDH3
-
 Vitis vinifera
1.1.1.25 VvSDH4
-
 Vitis vinifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.25 30
-
 assay at Vitis vinifera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.25 5.16
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 7.56
-
 shikimate pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 8.62
-
 3-dehydroshikimate pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 11.72
-
 shikimate pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 19.78
-
 NADP+ pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 26.11
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 26.12
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH4 Vitis vinifera
1.1.1.25 28.89
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH4 Vitis vinifera
1.1.1.25 30.7
-
 NADP+ pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 94.55
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 95.85
-
 3-dehydroshikimate pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 239
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.25 7
-
 shikimate dehydrogenase assay at, 3-dehydroshikimate production Vitis vinifera
1.1.1.25 9
-
 shikimate dehydrogenase assay at, gallate production Vitis vinifera

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.25 NADP+
-
 Vitis vinifera
1.1.1.25 NADPH
-
 Vitis vinifera

General Information

EC Number General Information Comment Organism
1.1.1.25 evolution phylogenetic analysis of VvSDH isozymes, isozyme VvSDH1 belongs to group I, and clusters with four characterized DQD/SDHs: AtSDH, Poptr1, JrSDH, and NtSDH1, group members share about 75% sequence identity Vitis vinifera
1.1.1.25 evolution phylogenetic analysis of VvSDH isozymes, isozyme VvSDH2 belongs to group II, and clusters with Poptr2 and Poptr3, two characterized DQD/SDHs, and NtSDH2, group members share about 71% sequence identity Vitis vinifera
1.1.1.25 evolution phylogenetic analysis of VvSDH isozymes, isozyme VvSDH3 belongs to group III, group members share about 85% sequence identity, four of them from different species (VvSDH3, CasSDH2, FvSDH1, and EgSDH3) accumulate gallic acid-based tannins Vitis vinifera
1.1.1.25 evolution phylogenetic analysis of VvSDH isozymes, isozyme VvSDH4 belongs to group IV, and clusters with with Poptr5, a DQD/SDH characterized in Populus trichocarpa, group members share about 77% sequence identity Vitis vinifera
1.1.1.25 metabolism in plants, the shikimate pathway provides aromatic amino acids that are used to generate numerous secondary metabolites, including phenolic compounds. In this pathway, shikimate dehydrogenases catalyse the reversible dehydrogenation of 3-dehydroshikimate to shikimate Vitis vinifera
1.1.1.25 metabolism in plants, the shikimate pathway provides aromatic amino acids that are used to generate numerous secondary metabolites, including phenolic compounds. In this pathway, shikimate dehydrogenases catalyse the reversible dehydrogenation of 3-dehydroshikimate to shikimate. Gallic acid metabolism in grape berry tissues along development, overview Vitis vinifera

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.25 18
-
 shikimate pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 76
-
 shikimate pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 178
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH4 Vitis vinifera
1.1.1.25 186
-
 3-dehydroshikimate pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 189
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 262
-
 NADP+ pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 537
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH4 Vitis vinifera
1.1.1.25 611
-
 3-dehydroshikimate pH 7.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 680
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 862
-
 NADP+ pH 7.0, 30°C, recombinant isozyme VvSDH3 Vitis vinifera
1.1.1.25 1051
-
 shikimate pH 9.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera
1.1.1.25 1113
-
 NADP+ pH 9.0, 30°C, recombinant isozyme VvSDH1 Vitis vinifera