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Literature summary extracted from

  • Xing, Y.; Chen, W.H.; Jia, W.; Zhang, J.
    Mitogen-activated protein kinase kinase 5 (MKK5)-mediated signalling cascade regulates expression of iron superoxide dismutase gene in Arabidopsis under salinity stress (2015), J. Exp. Bot., 66, 5971-5981.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.12.2 gene At3g21220, semi-quantitative reverse transcription-PCR expression analysis, cloning and expression of the GST-fusion enzyme MKK5 in Escherichia coli strain BL21 codon plus. Recombinant overexpression of DHA-tagged MKK5 under control of the CaMV 35S promoter in either Arabidopsis thaliana Col-0 wild-type plants or mkk5 plants Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.12.2 protoplast
-
 Arabidopsis thaliana
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.12.2 Mg2+ required Arabidopsis thaliana
2.7.12.2 NaCl mitogen-activated protein kinase kinase 5, MKK5, is activated in response to salt stress Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
2.7.12.2 Arabidopsis thaliana Q8RXG3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.12.2 seedling
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.12.2 ATP + myelin basic protein
-
 Arabidopsis thaliana ADP + phoshorylated myelin basic protein
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.12.2 At3g21220
-
 Arabidopsis thaliana
2.7.12.2 mitogen-activated protein kinase kinase 5
-
 Arabidopsis thaliana
2.7.12.2 MKK5
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.12.2 22
-
 assay at room temperature Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.12.2 7.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.12.2 ATP
-
 Arabidopsis thaliana

Expression

EC Number Organism Comment Expression
2.7.12.2 Arabidopsis thaliana construction of a kinase-dead enzyme mutant additional information

General Information

EC Number General Information Comment Organism
2.7.12.2 malfunction expression of iron superoxide dismutases FSD2 and FSD3 is significantly increased in Arabidopsis in response to NaCl treatment but blocked in transgenic MKK5-RNAi plant, mkk5. Overexpression of MKK5 in wild-type plants enhances their tolerance to salt treatments, while the mkk5 mutant exhibits hypersensitivity to salt stress in germination on salt-containing media. The kinase activity of MPK6 is totally turned off in mkk5, whereas the activity of MPK3 is only partially blocked. MKK5-overexpressing plants exhibit salt tolerance phenotypes Arabidopsis thaliana
2.7.12.2 metabolism te enzyme MKK5 is part of the MAP kinase cascade, including MEKK1, MKK5, and MPK6, that mediates the salt-induced expression of iron superoxide dismutases. MKK5 interactes with the MEKK1 protein that is also involved in the salt-induced FSD signalling pathway. Salt-induced iron superoxide dismutases FSD2 and FSD3 expressions are influenced by MEKK1 via MKK5-MPK6-coupled signalling. MPK6 is also involved in the MKK5-mediated iron superoxide dismutase signalling pathway in salt stress. The MKK5-MPK3/MPK6 pathway is similar to the flagellin-induced MAPK signalling pathway, MKK4/MKK5-MPK3/MPK6 Arabidopsis thaliana
2.7.12.2 physiological function the enzyme plays a role in MAPK signalling cascades involved in salt stress responses Arabidopsis thaliana