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Literature summary extracted from

  • Lou, S.S.; Diz-Munoz, A.; Weiner, O.D.; Fletcher, D.A.; Theriot, J.A.
    Myosin light chain kinase regulates cell polarization independently of membrane tension or Rho kinase (2015), J. Cell Biol., 209, 275-288.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.18 gene mylk5, expression analysis Danio rerio

Protein Variants

EC Number Protein Variants Comment Organism
2.7.11.18 additional information an antisense morpholino oligonucleotide is designed to induce mis-splicing of exon 9 of im:7148400 and early termination of the transcript before its kinase domain Danio rerio

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 ML-7
-
 Danio rerio

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Danio rerio F1QPV1
-
-
2.7.11.18 Danio rerio TL F1QPV1
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 embryo
-
 Danio rerio
-
2.7.11.18 keratocyte from 2 d or 4 d postfertilization (dpf) embryos, im:7148400 is the only MLCK isozyme in keratocytes Danio rerio
-

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 im:7148400
-
 Danio rerio
2.7.11.18 MLCK
-
 Danio rerio
2.7.11.18 mylk5
-
 Danio rerio
2.7.11.18 myosin light chain kinase
-
 Danio rerio

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Danio rerio

General Information

EC Number General Information Comment Organism
2.7.11.18 malfunction decreasing MLCK activity in 4-dpf cells results in longer edge lifetimes and wider edges. Enzyme inhibition causes no change in the lateral propagation rate, but an increase in edge lifetime, which suggests that MLCK activity regulates edge size by controlling edge lifetime Danio rerio
2.7.11.18 physiological function keratocytes from 2 d postfertilization (dpf) embryos resemble canonical fanshaped keratocytes, but keratocytes from 4 dpf embryos often form multiple protrusions despite unchanged membrane tension. 4-dpf Cells have multiple protrusions because the protrusions are intrinsically small. The multiple-protrusion phenotype is primarily due to increased myosin light chain kinase (MLCK) expression. MLCK activity influences cell polarity by increasing myosin accumulation in lamellipodia, which locally decreases protrusion lifetime, limiting lamellipodial size and allowing for multiple protrusions to coexist within the context of membrane tension limiting protrusion globally, while Rho kinase regulates myosin accumulation at the cell rear and does not determine protrusion size. MLCK-specific mechanism for controlling cell polarity via regulation of myosin activity in protrusions, overview. Enzyme inhibition causes no change in the lateral propagation rate, but an increase in edge lifetime, which suggests that MLCK activity regulates edge size by controlling edge lifetime Danio rerio