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Literature summary extracted from

  • Forsberg, Z.; Nelson, C.E.; Dalhus, B.; Mekasha, S.; Loose, J.S.; Crouch, L.I.; Rohr, A.K.; Gardner, J.G.; Eijsink, V.G.; Vaaje-Kolstad, G.
    Structural and functional analysis of a lytic polysaccharide monooxygenase important for efficient utilization of chitin in Cellvibrio japonicus (2016), J. Biol. Chem., 291, 7300-7312.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.53
-
 Cellvibrio japonicus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.53 to 1.85 A resolution, tri-modular enzyme containing a catalytic family AA10 LPMO module, a family 5 chitin-binding module, and a C-terminal unclassified module which displays tight and specific binding to chitin Cellvibrio japonicus

Protein Variants

EC Number Protein Variants Comment Organism
1.14.99.53 additional information removal of the chitin-binding modules reduces LPMO activity toward alpha-chitin. The full-length enzyme and the individual catalytic LPMO module boost the activity of an endochitinase equally well Cellvibrio japonicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.53 Cu2+ the active site contains a copper ion coordinated by residues His-37 and His-136 in a T-shaped histidine brace Cellvibrio japonicus

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.53 Cellvibrio japonicus B3PJ79
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.53 alpha-chitin + ascorbate + O2
-
 Cellvibrio japonicus chito-oligosaccharide aldonic acid + dehydroascorbate + H2O products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation ?
1.14.99.53 beta-chitin + acceptor + O2
-
 Cellvibrio japonicus chito-oligosaccharide aldonic acid + reduced acceptor + H2O products show a degree of polymerization from DP3 to DP8, with DP6 being the most abundant product after 24 h of incubation ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.53 Cbp33A
-
 Cellvibrio japonicus
1.14.99.53 LPMO10A
-
 Cellvibrio japonicus

General Information

EC Number General Information Comment Organism
1.14.99.53 physiological function the full-length enzyme and the individual catalytic LPMO module boost the activity of an endochitinase equally well, also yielding similar amounts of oxidized products. When grown on insoluble chitin substrates, the deletion mutant shows a reproducible growth defect on beta-chitin leading to an 2fold slower growth rate compared with the wild type strain. When grown on crab shells, the mutation leads to an extended lag phase of about 100 h Cellvibrio japonicus