Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Eibinger, M.; Ganner, T.; Bubner, P.; Rosker, S.; Kracher, D.; Haltrich, D.; Ludwig, R.; Plank, H.; Nidetzky, B.
    Cellulose surface degradation by a lytic polysaccharide monooxygenase and its effect on cellulase hydrolytic efficiency (2014), J. Biol. Chem., 289, 35929-35938.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.14.99.54 analysis assay based on adsorption of the fluorescent dye SYTO-62, adapted to identify and localize LPMO-catalyzed formation of carboxyl groups on a cellulose surface model substrate which provides amorphous and crystalline regions alternating on a nano-flat cellulose surface Neurospora crassa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.54 expression in Pichia pastoris Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Neurospora crassa Q1K4Q1
-
-
1.14.99.54 Neurospora crassa DSM 1257 Q1K4Q1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 avicel + ascorbate + O2
-
 Neurospora crassa ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 avicel + ascorbate + O2
-
 Neurospora crassa DSM 1257 ? + dehydroascorbate + H2O
-
 ?
1.14.99.54 cellulose + ascorbate + O2
-
 Neurospora crassa C1-oxidized cellooligosaccharides + dehydroascorbate + H2O
-
 ?
1.14.99.54 cellulose + ascorbate + O2
-
 Neurospora crassa DSM 1257 C1-oxidized cellooligosaccharides + dehydroascorbate + H2O
-
 ?

General Information

EC Number General Information Comment Organism
1.14.99.54 physiological function enzyme introduces carboxyl groups primarily in surface-exposed crystalline areas of the cellulosic substrate. Cellulose nano-fibrils exposed on the surface are degraded into shorter and thinner insoluble fragments. Prior action of LPMO enables cellulases to attack otherwise highly resistant crystalline substrate areas and promotes an overall faster and more complete surface degradation Neurospora crassa