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Literature summary extracted from

  • Tanaka, N.; Meineke, B.; Shuman, S.
    RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo (2011), J. Biol. Chem., 286, 30253-30257.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.5.1.8 expression in Saccharomyces cerevisiae Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.5.1.8 C78A almost complete loss of activity Escherichia coli
6.5.1.8 C78S complete loss of activity Escherichia coli
6.5.1.8 D75A almost complete loss of activity Escherichia coli
6.5.1.8 D75E complete loss of activity Escherichia coli
6.5.1.8 D75N complete loss of activity Escherichia coli
6.5.1.8 H168A almost complete loss of activity Escherichia coli
6.5.1.8 H168N complete loss of activity Escherichia coli
6.5.1.8 H168Q complete loss of activity Escherichia coli
6.5.1.8 H185A activity is severely impaired Escherichia coli
6.5.1.8 H280A active, protein is able to complement a Saccharomyces cerevisiae Trl1 mutant Escherichia coli
6.5.1.8 H337A almost complete loss of activity Escherichia coli
6.5.1.8 H337N complete loss of activity Escherichia coli
6.5.1.8 H337Q complete loss of activity Escherichia coli
6.5.1.8 K298A activity is severely impaired Escherichia coli
6.5.1.8 N167A activity is severely impaired Escherichia coli
6.5.1.8 R189A almost complete loss of activity Escherichia coli
6.5.1.8 R189A activity is severely impaired Escherichia coli
6.5.1.8 R341A almost complete loss of activity Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.5.1.8 Mn2+ required Escherichia coli
6.5.1.8 additional information Mg2+ cannot substitute for Mn2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
6.5.1.8 Escherichia coli P46850
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.5.1.8 (ribonucleotide)n-2',3'-cyclophosphate + 5'-hydroxy-(ribonucleotide)m + GTP + H2O substrate mimicks the broken tRNAGlu(UUC) anticodon stem-loop generated by Kluyveromyces lactis gamma-toxin, leaving 2',3'-cyclic phosphate and 5'-OH ends Escherichia coli (ribonucleotide)n+m + GMP + diphosphate overall reaction ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-hydroxy-(ribonucleotide)m + GTP
-
 Escherichia coli (ribonucleotide)n+m + GMP + diphosphate overall reaction, substrate 5'-hydroxy-(ribonucleotide)m can be composed of all ribonucleotides or all deoxynucleotides ?
6.5.1.8 (ribonucleotide)n-3'-phosphate + 5'-phospho-(ribonucleotide)m + GTP
-
 Escherichia coli (ribonucleotide)n+m + GMP + diphosphate overall reaction ?

General Information

EC Number General Information Comment Organism
6.5.1.8 physiological function RtcB is able in vivo to complement growth of yeast cells that lack the endogenous healing/sealing-type tRNA ligase Trl1. RtcB also protects yeast Trl1 mutant cells against a fungal ribotoxin that incises the anticodon loop of cellular tRNAs. RtcB can replace Trl1 as the catalyst of HAC1 mRNA splicing during the unfolded protein response Escherichia coli