BRENDA - Enzyme Database

Aprataxin forms a discrete branch in the HIT (histidine triad) superfamily of proteins with both DNA/RNA binding and nucleotide hydrolase activities

Kijas, A.W.; Harris, J.L.; Harris, J.M.; Lavin, M.F.; J. Biol. Chem. 281, 13939-13948 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.11.7
expression in Saccharomyces cerevisiae
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.11.7
additional information
removal of the N-terminal forkhead associated domain does not alter activity with substrates AMPNH2 and Ap4A
Homo sapiens
3.1.11.7
V263G
mutation identified in a AOA1 patient, mutant protein is unable to bind DNA
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.11.7
Duplex DNA
significantly reduces catalytic catalytic activity on the model histidine triad nucleotide-binding protein substrate, AMPNH2, and the Fragile histidine triad protein substrate, Ap4A
Homo sapiens
3.1.11.7
single-strand DNA
reduces catalytic catalytic activity on the model histidine triad nucleotide-binding protein substrate, AMPNH2, and the Fragile histidine triad protein substrate, Ap4A
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.11.7
0.018
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 7.0, 30°C
Homo sapiens
3.1.11.7
0.837
-
adenosine-5'-monophosphoramidate
pH 7.0, 30°C
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.11.7
Homo sapiens
Q7Z2E3
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.11.7
adenosine-5'-monophosphoramidate + H2O
-
740679
Homo sapiens
AMP + NH3
-
-
-
?
3.1.11.7
ATP + H2O
-
740679
Homo sapiens
AMP + diphosphate
-
-
-
?
3.1.11.7
additional information
aprataxin hydrolyzes with similar efficiency the model histidine triad nucleotide-binding protein substrate : adenosine-5'-monophosphoramidate, AMPNH2, and the Fragile histidine triad protein substrate, Ap4A. No substrate: dATP
740679
Homo sapiens
?
-
-
-
-
3.1.11.7
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
-
740679
Homo sapiens
AMP + ATP
-
-
-
?
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.11.7
0.0115
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 7.0, 30°C
Homo sapiens
3.1.11.7
0.0195
-
adenosine-5'-monophosphoramidate
pH 7.0, 30°C
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.11.7
expression in Saccharomyces cerevisiae
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.11.7
additional information
removal of the N-terminal forkhead associated domain does not alter activity with substrates AMPNH2 and Ap4A
Homo sapiens
3.1.11.7
V263G
mutation identified in a AOA1 patient, mutant protein is unable to bind DNA
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.11.7
Duplex DNA
significantly reduces catalytic catalytic activity on the model histidine triad nucleotide-binding protein substrate, AMPNH2, and the Fragile histidine triad protein substrate, Ap4A
Homo sapiens
3.1.11.7
single-strand DNA
reduces catalytic catalytic activity on the model histidine triad nucleotide-binding protein substrate, AMPNH2, and the Fragile histidine triad protein substrate, Ap4A
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.11.7
0.018
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 7.0, 30°C
Homo sapiens
3.1.11.7
0.837
-
adenosine-5'-monophosphoramidate
pH 7.0, 30°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.11.7
adenosine-5'-monophosphoramidate + H2O
-
740679
Homo sapiens
AMP + NH3
-
-
-
?
3.1.11.7
ATP + H2O
-
740679
Homo sapiens
AMP + diphosphate
-
-
-
?
3.1.11.7
additional information
aprataxin hydrolyzes with similar efficiency the model histidine triad nucleotide-binding protein substrate : adenosine-5'-monophosphoramidate, AMPNH2, and the Fragile histidine triad protein substrate, Ap4A. No substrate: dATP
740679
Homo sapiens
?
-
-
-
-
3.1.11.7
P1,P4-bis(5'-adenosyl)tetraphosphate + H2O
-
740679
Homo sapiens
AMP + ATP
-
-
-
?
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.1.11.7
0.0115
-
P1,P4-bis(5'-adenosyl)tetraphosphate
pH 7.0, 30°C
Homo sapiens
3.1.11.7
0.0195
-
adenosine-5'-monophosphoramidate
pH 7.0, 30°C
Homo sapiens
General Information
EC Number
General Information
Commentary
Organism
3.1.11.7
physiological function
aprataxin has dual DNA binding and nucleotide hydrolase activities. The protein binds to double-stranded DNA with high affinity but is also capable of binding double-stranded RNA and single-strand DNA, with increased affinity for hairpin structures. The DNA binding is not dependent on zinc
Homo sapiens
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.1.11.7
physiological function
aprataxin has dual DNA binding and nucleotide hydrolase activities. The protein binds to double-stranded DNA with high affinity but is also capable of binding double-stranded RNA and single-strand DNA, with increased affinity for hairpin structures. The DNA binding is not dependent on zinc
Homo sapiens