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Literature summary extracted from

  • Bhavsar, A.P.; Truant, R.; Brown, E.D.
    The TagB protein in Bacillus subtilis 168 is an intracellular peripheral membrane protein that can incorporate glycerol phosphate onto a membrane-bound acceptor in vitro (2005), J. Biol. Chem., 280, 36691-36700.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.44 expressed in Escherichia coli BL21 cells Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.44 H126A the mutant shows 75% less activity relative to the wild type enzyme Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.44 cytoplasmic membrane
-
 Bacillus subtilis
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.8.44 47079
-
-
 Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.44 CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Bacillus subtilis
-
 CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.44 CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol Bacillus subtilis 168
-
 CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.44 Bacillus subtilis P27621
-
-
2.7.8.44 Bacillus subtilis 168 P27621
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.8.44 HiTrap column chromatography and Superdex 200 gel filtration Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.44 CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Bacillus subtilis CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?
2.7.8.44 CDP-glycerol + N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 Bacillus subtilis 168 CDP + 4-O-((2R)-1-glycerophospho)-N-acetyl-beta-D-mannosaminyl-(1->4)-N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.8.44 ? x * 47079, calculated from amino acid sequence Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
2.7.8.44 CDP-glycerol:N-acetyl-beta-D-mannosaminyl-1,4-N-acetyl-D-glucosaminyldiphosphoundecaprenyl glycerophosphotransferase
-
 Bacillus subtilis
2.7.8.44 Tag primase
-
 Bacillus subtilis
2.7.8.44 tagB
-
 Bacillus subtilis