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Literature summary extracted from

  • Andersson, J.; Westman, M.; Hofer, A.; Sjoberg, B.M.
    Allosteric regulation of the class III anaerobic ribonucleotide reductase from bacteriophage T4 (2000), J. Biol. Chem., 275, 19443-19448.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.98.6 ATP positive effector for CTP reduction Tequatrovirus T4
1.1.98.6 dATP positive effector for CTP reduction Tequatrovirus T4
1.1.98.6 dGTP positive effector for ATP reduction Tequatrovirus T4
1.1.98.6 dTTP positive effector for GTP reduction Tequatrovirus T4
1.1.98.6 additional information enzyme has a single type of allosteric site to which dGTP, dTTP, dATP, and ATP bind competitively Tequatrovirus T4

Protein Variants

EC Number Protein Variants Comment Organism
1.1.98.6 N311A about 20% of wild-type activtiy Tequatrovirus T4
1.1.98.6 N311C less than 1% of wild-type activtiy Tequatrovirus T4
1.1.98.6 N78A less than 10% of wild-type activtiy Tequatrovirus T4
1.1.98.6 N78A/N311A less than 1% of wild-type activtiy Tequatrovirus T4
1.1.98.6 N78C 1% of wild-type activtiy Tequatrovirus T4
1.1.98.6 N78D 1% of wild-type activtiy Tequatrovirus T4

Organism

EC Number Organism UniProt Comment Textmining
1.1.98.6 Tequatrovirus T4 P07071
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.98.6 3.6
-
 mutant N78A/N311A, pH 8.0, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 5.6
-
 mutant N311C, pH 8.0, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 10.5
-
 mutant N78D, pH 8.0, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 11.8
-
 mutant N78C, pH 8.0, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 87.6
-
 mutant N78A, pH 8.0, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 207
-
 mutant N311A, pH 8.0, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 370
-
 substrate CTP, pH not specified in the publication, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 575
-
 substrate CTP, presence of ATP, pH not specified in the publication, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 730
-
 substrate CTP, presence of dATP, pH not specified in the publication, temperature not specified in the publication Tequatrovirus T4
1.1.98.6 1155
-
 wild-type, pH 8.0, temperature not specified in the publication Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.98.6 ATP + formate
-
 Tequatrovirus T4 dATP + CO2 + H2O
-
 ?
1.1.98.6 CTP + formate
-
 Tequatrovirus T4 dCTP + CO2 + H2O
-
 ?
1.1.98.6 GTP + formate
-
 Tequatrovirus T4 dGTP + CO2 + H2O
-
 ?
1.1.98.6 additional information no significant UTP reduction under the conditions used Tequatrovirus T4 ?
-
 ?
1.1.98.6 TTP + formate
-
 Tequatrovirus T4 dTTP + CO2 + H2O
-
 ?