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Literature summary extracted from

  • Shimotohno, K.; Seto, H.; Ōtake, N.; Imai, S.; Murakami, T.
    Studies on the biosynthesis of bialaphos (SF-1293). 8. Purification and characterization of 2-phosphinomethylmalic acid synthase from Streptomyces hygroscopicus SF-1293 (1988), J. Antibiot., 41, 1057-1065.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.18 4-chloromercuribenzoate 0.1 mM, 2% residual activity Streptomyces hygroscopicus
2.3.3.18 Ca2+ 1 mM, 54% residual activity Streptomyces hygroscopicus
2.3.3.18 EDTA 0.05 mM, almost complete inactivation Streptomyces hygroscopicus
2.3.3.18 Fe2+ 1 mM, 50% residual activity Streptomyces hygroscopicus
2.3.3.18 iodoacetic acid 1 mM, 18% residual activity Streptomyces hygroscopicus
2.3.3.18 Mg2+ 1 mM, 44% residual activity Streptomyces hygroscopicus
2.3.3.18 phosphate buffer not inhibitory at 10 mM, 50% loss of activity in 100 mM phosphate buffer Streptomyces hygroscopicus
2.3.3.18 Zn2+ 1 mM, 24% residual activity Streptomyces hygroscopicus
2.3.3.19 EDTA 0.05 mM, no residual activity. Activtity can be completely restored by addition of Co2+, or Mn2+ Streptomyces hygroscopicus
2.3.3.19 iodoacetamide 1 mM, 18% residual activity Streptomyces hygroscopicus
2.3.3.19 p-chloromercuribenzoate 0.1 mM, 2% residual activity Streptomyces hygroscopicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.18 0.052
-
 acetyl-CoA pH 8.0, 30°C Streptomyces hygroscopicus
2.3.3.18 0.13
-
 oxaloacetic acid pH 8.0, 30°C Streptomyces hygroscopicus
2.3.3.18 0.385
-
 3-(hydroxyphosphinoyl)pyruvate pH 8.0, 30°C Streptomyces hygroscopicus
2.3.3.19 0.05
-
 acetyl-CoA pH 7.5, 30°C Streptomyces hygroscopicus
2.3.3.19 0.13
-
 oxalacetic acid pH 7.5, 30°C Streptomyces hygroscopicus
2.3.3.19 0.39
-
 3-phosphonopyruvate pH 7.5, 30°C Streptomyces hygroscopicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.19 Co2+ addition completeley restores inactivation by EDTA Streptomyces hygroscopicus
2.3.3.19 Mn2+ addition completeley restores inactivation by EDTA Streptomyces hygroscopicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.18 48000
-
 2 * 48000, SDS-PAGE Streptomyces hygroscopicus
2.3.3.18 90000 98000 gel fitlration Streptomyces hygroscopicus
2.3.3.19 48000
-
 2 * 48000, SDS-PAGE Streptomyces hygroscopicus
2.3.3.19 90000 98000 gel filtration Streptomyces hygroscopicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.18 acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate Streptomyces hygroscopicus
-
 phosphinomethylmalate + CoA
-
 ?
2.3.3.18 acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate Streptomyces hygroscopicus SF-1293
-
 phosphinomethylmalate + CoA
-
 ?
2.3.3.18 acetyl-CoA + H2O + oxaloacetic acid Streptomyces hygroscopicus
-
 ? + CoA
-
 ?
2.3.3.18 acetyl-CoA + H2O + oxaloacetic acid Streptomyces hygroscopicus SF-1293
-
 ? + CoA
-
 ?
2.3.3.18 additional information Streptomyces hygroscopicus no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate ?
-
 ?
2.3.3.18 additional information Streptomyces hygroscopicus SF-1293 no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.18 Streptomyces hygroscopicus Q9LCB4
-
-
2.3.3.18 Streptomyces hygroscopicus SF-1293 Q9LCB4
-
-
2.3.3.19 Streptomyces hygroscopicus Q9LCB4
-
-
2.3.3.19 Streptomyces hygroscopicus SF-1293 Q9LCB4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.18
-
 Streptomyces hygroscopicus
2.3.3.19 from cell-free extract Streptomyces hygroscopicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.18 546.8
-
 pH 8.0, 30°C Streptomyces hygroscopicus

Storage Stability

EC Number Storage Stability Organism
2.3.3.18 4°C, lyophilized powder, 5% loss of activity within 3 months Streptomyces hygroscopicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.18 acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
 Streptomyces hygroscopicus phosphinomethylmalate + CoA
-
 ?
2.3.3.18 acetyl-CoA + H2O + 3-(hydroxyphosphinoyl)pyruvate
-
 Streptomyces hygroscopicus SF-1293 phosphinomethylmalate + CoA
-
 ?
2.3.3.18 acetyl-CoA + H2O + oxaloacetic acid
-
 Streptomyces hygroscopicus ? + CoA
-
 ?
2.3.3.18 acetyl-CoA + H2O + oxaloacetic acid
-
 Streptomyces hygroscopicus SF-1293 ? + CoA
-
 ?
2.3.3.18 additional information no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate Streptomyces hygroscopicus ?
-
 ?
2.3.3.18 additional information no substrates: propionyl-CoA or butanoyl-CoA and alpha-oxoacids such as pyruvate, 2-oxoglutarate, deamino-2-oxodemethylphosphinotricin or phosphonopyruvate Streptomyces hygroscopicus SF-1293 ?
-
 ?
2.3.3.19 acetyl-CoA + H2O + 3-phosphonopyruvate
-
 Streptomyces hygroscopicus (R)-2-(phosphonomethyl)malate + CoA
-
 ?
2.3.3.19 acetyl-CoA + H2O + 3-phosphonopyruvate
-
 Streptomyces hygroscopicus SF-1293 (R)-2-(phosphonomethyl)malate + CoA
-
 ?
2.3.3.19 acetyl-CoA + H2O + oxalacetic acid
-
 Streptomyces hygroscopicus ? + CoA
-
 ?
2.3.3.19 additional information no substrates: propionyl-CoA or butyryl-CoA, pyruvate, alpha-ketoglutarate, deamino-alpha-ketodemethylphosphinothricin or phosphonopyruvate Streptomyces hygroscopicus ?
-
 ?
2.3.3.19 additional information no substrates: propionyl-CoA or butyryl-CoA, pyruvate, alpha-ketoglutarate, deamino-alpha-ketodemethylphosphinothricin or phosphonopyruvate Streptomyces hygroscopicus SF-1293 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.18 dimer 2 * 48000, SDS-PAGE Streptomyces hygroscopicus
2.3.3.19 dimer 2 * 48000, SDS-PAGE Streptomyces hygroscopicus

Synonyms

EC Number Synonyms Comment Organism
2.3.3.18 2-phosphinomethylmalic acid synthase
-
 Streptomyces hygroscopicus
2.3.3.18 pmmS
-
 Streptomyces hygroscopicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.18 30
-
-
 Streptomyces hygroscopicus
2.3.3.19 30
-
-
 Streptomyces hygroscopicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.18 8
-
-
 Streptomyces hygroscopicus
2.3.3.19 8
-
-
 Streptomyces hygroscopicus