BRENDA - Enzyme Database

Biochemical characterization of the tetrachlorobenzoquinone reductase involved in the biodegradation of pentachlorophenol

Chen, L.; Yang, J.; Int. J. Mol. Sci. 9, 198-212 (2008)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.404
tetrachlorohydroquinone
activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone
Sphingobium chlorophenolicum
Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.404
expression in Eshcerichia coli
Sphingobium chlorophenolicum
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.1.1.404
homology modeling of structure. The active site is slightly positively charged and situated in a deep pit on the surface. The putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster. It is formed mainly by residues Ser78, Arg79, Phe225, Gly226, Ala227, Ala228, Leu229, Gln275 and the cofactor FMN
Sphingobium chlorophenolicum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.404
Pentachlorophenol
competitive, 55% loss of activity at 0.1 mM
Sphingobium chlorophenolicum
1.1.1.404
tetrachlorohydroquinone
activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone
Sphingobium chlorophenolicum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.404
Iron
protein contains 1.7 mol of iron per mol
Sphingobium chlorophenolicum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.404
additional information
-
protein exists as trimer, dynamic light scattering
Sphingobium chlorophenolicum
1.1.1.404
36000
-
-
Sphingobium chlorophenolicum
1.1.1.404
36500
-
-
Sphingobium chlorophenolicum
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.404
Sphingobium chlorophenolicum
Q47914
-
-
1.1.1.404
Sphingobium chlorophenolicum ATCC 39723
Q47914
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.404
recombinant protein,. Purified enzyme is colorless
Sphingobium chlorophenolicum
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.404
2.1
-
pH 7.0, 25░C
Sphingobium chlorophenolicum
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.404
2,3,5,6-tetrachlorohydroquinone + NAD+
-
740552
Sphingobium chlorophenolicum
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
-
-
?
1.1.1.404
2,3,5,6-tetrachlorohydroquinone + NAD+
-
740552
Sphingobium chlorophenolicum ATCC 39723
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.1.1.404
homotrimer
3 * 36000, SDS-PAGE, 3 * 36500, mass spectrometry, 3 * 36500, calculated
Sphingobium chlorophenolicum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.404
7
-
-
Sphingobium chlorophenolicum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.404
5
-
70% of maximum activity
Sphingobium chlorophenolicum
1.1.1.404
8
-
20% of maximum activity
Sphingobium chlorophenolicum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.404
FMN
the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster
Sphingobium chlorophenolicum
1.1.1.404
[2Fe-2S]-center
the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster
Sphingobium chlorophenolicum
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.1.1.404
tetrachlorohydroquinone
activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone
Sphingobium chlorophenolicum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.404
expression in Eshcerichia coli
Sphingobium chlorophenolicum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.404
FMN
the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster
Sphingobium chlorophenolicum
1.1.1.404
[2Fe-2S]-center
the putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster
Sphingobium chlorophenolicum
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.1.1.404
homology modeling of structure. The active site is slightly positively charged and situated in a deep pit on the surface. The putative binding pocket is adjacent to the cofactor flavin mononucleotide and the 2Fe-2S cluster. It is formed mainly by residues Ser78, Arg79, Phe225, Gly226, Ala227, Ala228, Leu229, Gln275 and the cofactor FMN
Sphingobium chlorophenolicum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.404
Pentachlorophenol
competitive, 55% loss of activity at 0.1 mM
Sphingobium chlorophenolicum
1.1.1.404
tetrachlorohydroquinone
activity of PcpD is stimulated by tetrachlorohydroquinone at low concentrations but inhibited at high concentrations. 83% increase in activity at 0.1 mM tetrachlorohydroquinone, 60% loss of activity at 0.2 mM tetrachlorohydroquinone
Sphingobium chlorophenolicum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.1.1.404
Iron
protein contains 1.7 mol of iron per mol
Sphingobium chlorophenolicum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.1.1.404
additional information
-
protein exists as trimer, dynamic light scattering
Sphingobium chlorophenolicum
1.1.1.404
36000
-
-
Sphingobium chlorophenolicum
1.1.1.404
36500
-
-
Sphingobium chlorophenolicum
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.404
recombinant protein,. Purified enzyme is colorless
Sphingobium chlorophenolicum
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
1.1.1.404
2.1
-
pH 7.0, 25░C
Sphingobium chlorophenolicum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.404
2,3,5,6-tetrachlorohydroquinone + NAD+
-
740552
Sphingobium chlorophenolicum
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
-
-
?
1.1.1.404
2,3,5,6-tetrachlorohydroquinone + NAD+
-
740552
Sphingobium chlorophenolicum ATCC 39723
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.1.1.404
homotrimer
3 * 36000, SDS-PAGE, 3 * 36500, mass spectrometry, 3 * 36500, calculated
Sphingobium chlorophenolicum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.404
7
-
-
Sphingobium chlorophenolicum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.1.404
5
-
70% of maximum activity
Sphingobium chlorophenolicum
1.1.1.404
8
-
20% of maximum activity
Sphingobium chlorophenolicum