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Literature summary extracted from

  • Eronina, T.B.; Chebotareva, N.A.; Sluchanko, N.N.; Mikhaylova, V.V.; Makeeva, V.F.; Roman, S.G.; Kleymenov, S.Y.; Kurganov, B.I.
    Dual effect of arginine on aggregation of phosphorylase kinase (2014), Int. J. Biol. Macromol., 68, 225-232.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ca2+ dependent on Oryctolagus cuniculus
2.7.11.19 Mg2+ required Oryctolagus cuniculus
2.7.11.19 additional information Ca2+ and Mg2+ induce self-aggregation of PhK at 37°C Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.19 2 ATP + glycogen phosphorylase b Oryctolagus cuniculus
-
 2 ADP + glycogen phosphorylase a
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus P18688 AND P12798 subunits a and b
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 2 ATP + glycogen phosphorylase b
-
 Oryctolagus cuniculus 2 ADP + glycogen phosphorylase a
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.19 hexadecamer (alphabetagammadelta)4 Oryctolagus cuniculus
2.7.11.19 More Ca2+and Mg2+ions induce the self-association of PhK. Effects of arginine on protein-protein interactions in the enzyme polymer: arginine induces aggregation of Ca2+-free enzyme PhK. But when studying Ca2+, Mg2+-induced aggregation of PhK at 37°C, the protective effect of arginine is demon-strated, disruption of PhK hexadecameric structure occurs under the action of arginine. Although HspB6 and HspB5 suppress aggregation of PhK they do not block the disruption effect of arginine with respect to both forms of PhK (Ca2+-free and Ca2+, Mg2+-bound conformers). Aggregation of PhK induced by 0.5 M Arg is significantly suppressed in the presence of 1 M trimethylamine N-oxide dihydrate (TMAO) Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.19 PhK
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 25 37 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 6.8
-
 assay at Oryctolagus cuniculus

General Information

EC Number General Information Comment Organism
2.7.11.19 physiological function the enzyme is a Ca2+-dependent regulatory enzymethat catalyzes phosphorylation and activation of glycogen phosphorylase b Oryctolagus cuniculus