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Literature summary extracted from

  • Telke, A.A.; Rolain, J.M.
    Functional genomics to discover antibiotic resistance genes: the paradigm of resistance to colistin mediated by ethanolamine phosphotransferase in Shewanella algae MARS 14 (2015), Int. J. Antimicrob. Agents, 46, 648-652.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.43 gene eptA, construction of a pZE21MCS-1 plasmid-based genomic expression library in Escherichia coli strain TOP10, DNA and amino acid sequence determination and analysis, functional EptA enzyme expression in Escherichia coli strain TOP10 Shewanella algae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.43 diacylphosphatidylethanolamine + lipid A Shewanella algae
-
 diacylglycerol + lipid A (2-aminoethyl diphosphate)
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A Shewanella algae MARS 14
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 diacylglycerol + lipid A (2-aminoethyl diphosphate)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.43 Shewanella algae A0A0H5BY27 a colistin-resistant clinical isolate from resistant clinical isolate retrieved from bronchoalveolar lavage of a hospitalised patient
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2.7.8.43 Shewanella algae MARS 14 A0A0H5BY27 a colistin-resistant clinical isolate from resistant clinical isolate retrieved from bronchoalveolar lavage of a hospitalised patient
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.43 diacylphosphatidylethanolamine + lipid A
-
 Shewanella algae diacylglycerol + lipid A (2-aminoethyl diphosphate)
-
 ?
2.7.8.43 diacylphosphatidylethanolamine + lipid A
-
 Shewanella algae MARS 14 diacylglycerol + lipid A (2-aminoethyl diphosphate)
-
 ?
2.7.8.43 additional information lipid A substrate and product analysis by MALDI-TOF/MS. The lipidA preparations from transgenic Escherichia coli strains carrying EptA show additional ions due to the addition of phosphoethanolamine to the bis-phosphorylatedstructure and the hepta-acylated structure Shewanella algae ?
-
 ?
2.7.8.43 additional information lipid A substrate and product analysis by MALDI-TOF/MS. The lipidA preparations from transgenic Escherichia coli strains carrying EptA show additional ions due to the addition of phosphoethanolamine to the bis-phosphorylatedstructure and the hepta-acylated structure Shewanella algae MARS 14 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.43 EptA
-
 Shewanella algae
2.7.8.43 ethanolamine phosphotransferase
-
 Shewanella algae
2.7.8.43 PmrC
-
 Shewanella algae

General Information

EC Number General Information Comment Organism
2.7.8.43 physiological function the paradigm of resistance to antibiotic colistin mediated by ethanolamine phosphotransferase in Shewanella algae MARS 14. Resistance to colistin in Shewanella algae MARS 14 is associated with overexpression of enzyme EptA (27fold increase), which plays a crucial role in the arrangement of outer membrane lipopolysaccharide Shewanella algae