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Literature summary extracted from

  • Horstmann, N.; Sahasrabhojane, P.; Saldana, M.; Ajami, N.J.; Flores, A.R.; Sumby, P.; Liu, C.G.; Yao, H.; Su, X.; Thompson, E.; Shelburne, S.A.
    Characterization of the effect of the histidine kinase CovS on response regulator phosphorylation in group A Streptococcus (2015), Infect. Immun., 83, 1068-1077.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.13.3 gene covS, dNA and amino acid sequence determination and analysis Streptococcus pyogenes MGAS10870

Protein Variants

EC Number Protein Variants Comment Organism
2.7.13.3 E281A site-directed mutagenesis Streptococcus pyogenes MGAS10870
2.7.13.3 G457V site-directed mutagenesis Streptococcus pyogenes MGAS10870
2.7.13.3 H280V site-directed mutagenesis Streptococcus pyogenes MGAS10870
2.7.13.3 I332V site-directed mutagenesis Streptococcus pyogenes MGAS10870
2.7.13.3 additional information genetic inactivation of covS decreases but does not eliminate CovR phosphorylation Streptococcus pyogenes MGAS10870
2.7.13.3 T284A site-directed mutagenesis Streptococcus pyogenes MGAS10870

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.13.3 Mg2+ required Streptococcus pyogenes MGAS2221
2.7.13.3 Mg2+ required, activates CovR phosphorylation at high concentration. High-Mg2+ conditions increase phosphorylated CovR levels dependent on the presence of an intact CovS protein without influencing CovR production Streptococcus pyogenes MGAS10870

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.13.3 ATP + CovR L-histidine Streptococcus pyogenes MGAS10870
-
 ADP + CovR N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + CovR L-histidine Streptococcus pyogenes MGAS2221
-
 ADP + CovR N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + protein L-histidine Streptococcus pyogenes MGAS10870
-
 ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + protein L-histidine Streptococcus pyogenes MGAS2221
-
 ADP + protein N-phospho-L-histidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.13.3 Streptococcus pyogenes MGAS10870
-
 serotype M3
-
2.7.13.3 Streptococcus pyogenes MGAS2221
-
 serotype M1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.13.3 ATP + CovR L-histidine
-
 Streptococcus pyogenes MGAS10870 ADP + CovR N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + CovR L-histidine
-
 Streptococcus pyogenes MGAS2221 ADP + CovR N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + protein L-histidine
-
 Streptococcus pyogenes MGAS10870 ADP + protein N-phospho-L-histidine
-
 ?
2.7.13.3 ATP + protein L-histidine
-
 Streptococcus pyogenes MGAS2221 ADP + protein N-phospho-L-histidine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.13.3 CovS
-
 Streptococcus pyogenes MGAS10870
2.7.13.3 CovS
-
 Streptococcus pyogenes MGAS2221

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.13.3 ATP
-
 Streptococcus pyogenes MGAS10870
2.7.13.3 ATP
-
 Streptococcus pyogenes MGAS2221

General Information

EC Number General Information Comment Organism
2.7.13.3 malfunction a group A streptococci strain selectively deficient in CovS phosphatase activity has a distinct transcriptome relative to that of its parental strain. Inactivation of CovS in the serotype M1 background results in a greater decrease in phosphorylated CovR levels and a greater increase in the transcript levels of CovR-repressed genes than does CovS inactivation in a serotype M3 strain Streptococcus pyogenes MGAS10870
2.7.13.3 malfunction a group A streptococci strain selectively deficient in CovS phosphatase activity has a distinct transcriptome relative to that of its parental strain. Inactivation of CovS in the serotype M1 background results in a greater decrease in phosphorylated CovR levels and a greater increase in the transcript levels of CovR-repressed genes than does CovS inactivation in a serotype M3 strain Streptococcus pyogenes MGAS2221
2.7.13.3 metabolism compared to a serotype M3 strain, serotype M1 GAS strains have high levels of phosphorylated CovR, low transcript levels of CovR-repressed genes, and strikingly different responses to environmental cues Streptococcus pyogenes MGAS2221
2.7.13.3 metabolism compared to a serotype M3 strain, serotype M1 GAS strains have high levels of phosphorylated CovR, low transcript levels of CovR-repressed genes, and strikingly different responses to environmental cues. Genetic inactivation of covS decreases but does not eliminate CovR phosphorylation Streptococcus pyogenes MGAS10870
2.7.13.3 physiological function two-component gene regulatory systems (TCSs) are a major mechanism by which bacteria respond to environmental stimuli and are critical to infectivity. The control of virulence regulator/sensor kinase (CovRS) TCS is central to the virulence of the major human pathogen group A Streptococcus (GAS). In the system, the histidine kinase CovS primarily serves to phosphorylate CovR, thereby resulting in the repression of virulence factor-encoding genes. Both CovS kinase and phosphatase activities influence the CovR phosphorylation status. Serotype M1 GAS strains have high rates of spontaneous mutations in covS during invasive GAS infection, thus providing a link between TCS molecular function and the epidemiology of deadly bacterial infections Streptococcus pyogenes MGAS10870
2.7.13.3 physiological function two-component gene regulatory systems (TCSs) are a major mechanism by which bacteria respond to environmental stimuli and are critical to infectivity. The control of virulence regulator/sensor kinase (CovRS) TCS is central to the virulence of the major human pathogen group A Streptococcus (GAS). In the system, the histidine kinase CovS primarily serves to phosphorylate CovR, thereby resulting in the repression of virulence factor-encoding genes. Both CovS kinase and phosphatase activities influence the CovR phosphorylation status. Serotype M1 GAS strains have high rates of spontaneous mutations in covS during invasive GAS infection, thus providing a link between TCS molecular function and the epidemiology of deadly bacterial infections Streptococcus pyogenes MGAS2221