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Literature summary extracted from

  • Kyprianou, P.; Betteridge, A.; Donald, A.S.; Watkins, W.M.
    Purification of the blood group H gene associated alpha-2-L-fucosyltransferase from human plasma (1990), Glycoconj. J., 7, 573-588.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.69 beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose i.e. lacto-N-neotetraose, competitive with respect to lacto-N-biose I Homo sapiens
2.4.1.344 beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose i.e. lacto-N-tetraose, competitive with respect to N-acetyllactosamine Homo sapiens
2.4.1.344 beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose i.e. lacto-N-neotetraose, competitive with respect to lacto-N-biose I Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.69 4.5
-
 beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine pH 7.0, 37°C Homo sapiens
2.4.1.344 3.4
-
 beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose pH 7.0, 37°C Homo sapiens
2.4.1.344 4
-
 phenyl beta-D-galactopyranoside pH 7.0, 37°C Homo sapiens
2.4.1.344 5
-
 beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose pH 7.0, 37°C Homo sapiens
2.4.1.344 5
-
 beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine pH 7.0, 37°C Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.69 158000
-
 gel filtration Homo sapiens
2.4.1.344 158000
-
 gel filtration Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Homo sapiens
-
-
-
2.4.1.344 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.69 purification from plasma Homo sapiens
2.4.1.344 purification from plasma Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.69 blood plasma
-
 Homo sapiens
-
2.4.1.344 blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose I, reaction of EC 2.4.1.69 Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine 82% of the activity with phenyl beta-D-galactopyranoside ?
2.4.1.69 additional information the enzyme transfers fucose equally well to type I (Galbeta-(1->3)-GIcNAc) and type 2 (Galbeta-(1->4)-GIcNAc) substrates, reaction of EC 2.4.1.344, but type 3 (Galbeta-(1->3)-GalNAc) structures are less efficient acceptors Homo sapiens ?
-
 ?
2.4.1.344 GDP-beta-L-fucose + 2-nitrophenyl beta-D-galactopyranoside
-
 Homo sapiens GDP + 2-nitrophenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside 137% of the activity with phenyl beta-D-galactopyranoside ?
2.4.1.344 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine i.e. lacto-N-biose I, reaction of EC 2.4.1.69 Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-D-glucosamine 82% of the activity with phenyl beta-D-galactopyranoside ?
2.4.1.344 GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose i.e. lacto-N-tetraose, residues of H type 1 epitopes, reaction of EC 2.4.1.69 Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acteyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose 131% of the activity with phenyl beta-D-galactopyranoside ?
2.4.1.344 GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine i.e. N-acetyllactosamine Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosamine 80% of the activity with phenyl beta-D-galactopyranoside ?
2.4.1.344 GDP-beta-L-fucose + beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose i.e. lacto-N-neotetraose, residues of H type 2 epitopes Homo sapiens GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->4)-N-acetyl-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-D-glucose 113% of the activity with phenyl beta-D-galactopyranoside ?
2.4.1.344 GDP-beta-L-fucose + phenyl beta-D-galactopyranoside
-
 Homo sapiens GDP + phenyl alpha-L-fucosyl-(1->2)-beta-D-galactopyranoside
-
 ?
2.4.1.344 additional information the enzyme transfers fucose equally well to type I (Galbeta-(1->3)-GIcNAc) and type 2 (Galbeta-(1->4)-GIcNAc) substrates but type 3 (Galbeta-(1->3)-GalNAc) structures are less efficient acceptors Homo sapiens ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.69 6.5 7
-
 Homo sapiens
2.4.1.344 6.5 7
-
 Homo sapiens