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Literature summary extracted from

  • Justino, M.C.; Parente, M.R.; Boneca, I.G.; Saraiva, L.M.
    FrxA is an S-nitrosoglutathione reductase enzyme that contributes to Helicobacter pylori pathogenicity (2014), FEBS J., 281, 4495-4505.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.284 0.0041
-
 S-nitrosoglutathione pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.0072
-
 S-nitrosocysteine pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.0143
-
 nitrofurazone pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.0224
-
 metronidazole pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.0423
-
 Nitrofurantoin pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.0626
-
 3-nitrotyrosine pH 7.5, 37°C Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.284 26000
-
 2 * 26000, SDS-PAGE Helicobacter pylori
1.1.1.284 52000
-
 gel filtration Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.284 Helicobacter pylori
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.284 3-nitrotyrosine + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
1.1.1.284 metronidazole + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
1.1.1.284 nitrofurantoin + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
1.1.1.284 nitrofurazone + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
1.1.1.284 S-nitrosocysteine + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?
1.1.1.284 S-nitrosoglutathione + NADPH + H+
-
 Helicobacter pylori ? + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.284 dimer 2 * 26000, SDS-PAGE Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
1.1.1.284 FrxA
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.284 0.1
-
 3-nitrotyrosine pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.5
-
 S-nitrosoglutathione pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.5
-
 Nitrofurantoin pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.6
-
 metronidazole pH 7.5, 37°C Helicobacter pylori
1.1.1.284 0.6
-
 S-nitrosocysteine pH 7.5, 37°C Helicobacter pylori
1.1.1.284 1
-
 nitrofurazone pH 7.5, 37°C Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.284 FMN 0.2 FMN molecules per FrxA polypeptide chain Helicobacter pylori

Expression

EC Number Organism Comment Expression
1.1.1.284 Helicobacter pylori transcription of FrxA is induced by nitrosative stress up

General Information

EC Number General Information Comment Organism
1.1.1.284 physiological function inactivation of the FrxA-encoding gene results in a strain more sensitive to S-nitrosoglutathione. FrxA contributes to the proliferation of Helicobacter pylori in macrophages and FrxA supports the virulence of the pathogen upon mouse infection Helicobacter pylori

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.284 2.7
-
 3-nitrotyrosine pH 7.5, 37°C Helicobacter pylori
1.1.1.284 11
-
 Nitrofurantoin pH 7.5, 37°C Helicobacter pylori
1.1.1.284 25
-
 metronidazole pH 7.5, 37°C Helicobacter pylori
1.1.1.284 71
-
 nitrofurazone pH 7.5, 37°C Helicobacter pylori
1.1.1.284 89
-
 S-nitrosocysteine pH 7.5, 37°C Helicobacter pylori
1.1.1.284 126
-
 S-nitrosoglutathione pH 7.5, 37°C Helicobacter pylori