Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yu, M.J.; Yoon, S.H.; Kim, Y.W.
    Overproduction and characterization of a lytic polysaccharide monooxygenase in Bacillus subtilis using an assay based on ascorbate consumption (2016), Enzyme Microb. Technol., 93-94, 150-156.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.99.53 analysis development of a chitinase-coupled assay method and an assay based on consumption of ascorbate. There is no H2O2 production in the presence of colloidal chitin at 4 mg/ml Bacillus atrophaeus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.53 expression in Baciluus subtilis Bacillus atrophaeus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.53 NaCl enzyme activity is decreased by NaCl Bacillus atrophaeus

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.53 Bacillus atrophaeus A0A0H3E2X6
-
-
1.14.99.53 Bacillus atrophaeus 1942 A0A0H3E2X6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.53 expression Bacillus subtilis yields a simple purification process without complicated periplasmic fractionation, as well as improved productivity Bacillus atrophaeus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.53 colloidal chitin + ascorbate + O2
-
 Bacillus atrophaeus [chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O
-
 ?
1.14.99.53 colloidal chitin + ascorbate + O2
-
 Bacillus atrophaeus 1942 [chitin oligosaccharide]-N-acetyl-D-glucosaminate + [chitin oligosaccharide]-N-acetyl-D-glucosamino-1,5-lactone + dehydroascorbate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.53 BATR1942_08650
-
 Bacillus atrophaeus
1.14.99.53 LPMO10
-
 Bacillus atrophaeus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.99.53 8
-
 Tris/HCl buffer Bacillus atrophaeus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.99.53 6
-
 ammonium acetate buffer, 34% of maximum activity Bacillus atrophaeus

General Information

EC Number General Information Comment Organism
1.14.99.53 physiological function LPMO10 and a chitinase mutually enhance each otherย’s activities upon degrading chitin as the substrate Bacillus atrophaeus