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Literature summary extracted from

  • Liu, A.; Feng, R.; Liang, B.
    Microbial surface displaying formate dehydrogenase and its application in optical detection of formate (2016), Enzyme Microb. Technol., 91, 59-65.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.9 expressed in Escherichia coli Transetta (DE3) cells [Candida] boidinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 Ca2+ 61.51% residual activity at 10 mM [Candida] boidinii
1.17.1.9 CO32- 16.93% residual activity at 100 mM [Candida] boidinii
1.17.1.9 H2PO4- 78.54% residual activity at 100 mM [Candida] boidinii
1.17.1.9 Mg2+ 89.93% residual activity at 10 mM [Candida] boidinii
1.17.1.9 NO3- 40.89% residual activity at 100 mM [Candida] boidinii
1.17.1.9 Pb2+ 24.33% residual activity at 10 mM [Candida] boidinii
1.17.1.9 PO43- 30.9% residual activity at 100 mM [Candida] boidinii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.17.1.9 additional information Li+, Na+, K+, NH4+, Cl?, HPO42-, HCO3-, and SO42- at 100 mM have scarcely effect on the enzymatic activity. The enzyme activity is very slightly increased in the presence of 1 mM Co2+, Ni2+, Cu2+, Zn2+, Fe3+, Mn2+, Mg2+, Ca2+, and NH4+ [Candida] boidinii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.17.1.9 100000
-
-
 [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 [Candida] boidinii O13437
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?
1.17.1.9 additional information no activity with methanol, formaldehyde, ethanol, sodium acetate, sodium propionate, sodium oxalate, and sodium citrate [Candida] boidinii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.17.1.9 ? x * 100000, enzyme fused to maltose binding protein and ice nucleation protein, SDS-PAGE [Candida] boidinii

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 FDH
-
 [Candida] boidinii
1.17.1.9 NAD+-dependent formate dehydrogenase
-
 [Candida] boidinii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.17.1.9 50
-
-
 [Candida] boidinii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.17.1.9 40 60 more than 40% activity between 40 and 60°C [Candida] boidinii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.17.1.9 4 50 the Escherichia coli cell-surface-displayed enzyme retains its original enzymatic activity after incubation at 4°C for 1 month with the half-life of 17 days at 40°C and 38 h at 50°C [Candida] boidinii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.17.1.9 9
-
-
 [Candida] boidinii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.17.1.9 6 10 more than 50% activity between pH 6.0 and 10.0 [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 [Candida] boidinii