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Literature summary extracted from

  • Leissing, F.; Nomoto, M.; Bocola, M.; Schwaneberg, U.; Tada, Y.; Conrath, U.; Beckers, G.J.
    Substrate thiophosphorylation by Arabidopsis mitogen-activated protein kinases (2016), BMC Plant Biol., 16, 48.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.24 additional information the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana
2.7.11.24 additional information the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana
2.7.11.24 additional information the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.24 recombinant expression of GST-tagged MPK3 in Escherichia coli Arabidopsis thaliana
2.7.11.24 recombinant expression of GST-tagged MPK4 in Escherichia coli Arabidopsis thaliana
2.7.11.24 recombinant expression of GST-tagged MPK6 in Escherichia coli Arabidopsis thaliana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.24 Mg2+ required Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.24 ATP + Arabidopsis thaliana protein AT1G7815 Arabidopsis thaliana
-
 ADP + phosphorylated Arabidopsis thaliana proteins AT1G78150
-
 ?
2.7.11.24 ATP + Arabidopsis thaliana protein AT2G26530 Arabidopsis thaliana
-
 ADP + phosphorylated Arabidopsis thaliana proteins AT2G26530
-
 ?
2.7.11.24 ATP + Arabidopsis thaliana protein AT3G11330 Arabidopsis thaliana
-
 ADP + phosphorylated Arabidopsis thaliana proteins AT3G11330
-
 ?
2.7.11.24 ATP + Arabidopsis thaliana protein AT4G38710 Arabidopsis thaliana
-
 ADP + phosphorylated Arabidopsis thaliana proteins AT4G38710
-
 ?
2.7.11.24 ATP + myelin basic protein Arabidopsis thaliana
-
 ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.24 ATPgammaS + myelin basic protein Arabidopsis thaliana Arabidopsis thaliana MPKs use ATPgammaS to thiophosphorylate myelin basic protein ADP + thiophosphorylated myelin basic protein
-
 ?
2.7.11.24 additional information Arabidopsis thaliana FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4 ?
-
 ?
2.7.11.24 N6-benzyl-ATPgammaS + myelin basic protein Arabidopsis thaliana Arabidopsis thaliana MPK3 mutant T119A uses N6-benzyl-ATPgammaS to thiophosphorylate myelin basic protein ADP + benzylthiophosphorylated myelin basic protein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.24 Arabidopsis thaliana Q39023
-
-
2.7.11.24 Arabidopsis thaliana Q39024
-
-
2.7.11.24 Arabidopsis thaliana Q39026
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.24 phosphoprotein the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK3 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana
2.7.11.24 phosphoprotein the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK4 is activated by constitutively active MKK1 and MKK2, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana
2.7.11.24 phosphoprotein the enzyme is phosphorylated and activated by a mitogen-activated protein kinase kinase, GST-tagged MPK6 is activated by constitutively active MKK4 and MKK5, which cause a dual phosphorylation of the TEY motif in the activation loop Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.24 recombinant GST-tagged MPK3 by affinity chromatography from Escherichia coli Arabidopsis thaliana
2.7.11.24 recombinant GST-tagged MPK4 by affinity chromatography from Escherichia coli Arabidopsis thaliana
2.7.11.24 recombinant GST-tagged MPK6 by affinity chromatography from Escherichia coli Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.24 ATP + Arabidopsis thaliana protein AT1G7815
-
 Arabidopsis thaliana ADP + phosphorylated Arabidopsis thaliana proteins AT1G78150
-
 ?
2.7.11.24 ATP + Arabidopsis thaliana protein AT2G26530
-
 Arabidopsis thaliana ADP + phosphorylated Arabidopsis thaliana proteins AT2G26530
-
 ?
2.7.11.24 ATP + Arabidopsis thaliana protein AT3G11330
-
 Arabidopsis thaliana ADP + phosphorylated Arabidopsis thaliana proteins AT3G11330
-
 ?
2.7.11.24 ATP + Arabidopsis thaliana protein AT4G38710
-
 Arabidopsis thaliana ADP + phosphorylated Arabidopsis thaliana proteins AT4G38710
-
 ?
2.7.11.24 ATP + myelin basic protein
-
 Arabidopsis thaliana ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.24 ATPgammaS + myelin basic protein Arabidopsis thaliana MPKs use ATPgammaS to thiophosphorylate myelin basic protein Arabidopsis thaliana ADP + thiophosphorylated myelin basic protein
-
 ?
2.7.11.24 additional information FLAG-tagged Arabidopsis thaliana proteins AT1G78150, AT2G26530, AT3G11330, and AT4G38710 are good MPK3/6 substrates, but are poor substrates for the closely related Arabidopsis thaliana MPK4 Arabidopsis thaliana ?
-
 ?
2.7.11.24 N6-benzyl-ATPgammaS + myelin basic protein Arabidopsis thaliana MPK3 mutant T119A uses N6-benzyl-ATPgammaS to thiophosphorylate myelin basic protein Arabidopsis thaliana ADP + benzylthiophosphorylated myelin basic protein
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.24 More residue Y119 is the gatekeeper in MPK3, three-dimensional structure analysis Arabidopsis thaliana
2.7.11.24 More residue Y124 is the gatekeeper in MPK4, three-dimensional structure analysis Arabidopsis thaliana
2.7.11.24 More residue Y144 is the gatekeeper in MPK6, three-dimensional structure analysis Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
2.7.11.24 MPK3
-
 Arabidopsis thaliana
2.7.11.24 MPK4
-
 Arabidopsis thaliana
2.7.11.24 MPK6
-
 Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.24 7.5
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.24 ATP
-
 Arabidopsis thaliana
2.7.11.24 ATPgammaS MPK3 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins Arabidopsis thaliana
2.7.11.24 ATPgammaS MPK4 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins Arabidopsis thaliana
2.7.11.24 ATPgammaS MPK6 accepts ATPgammaS as a phosphoryldonor to thiophosphorylate myelin basic protein and supposedly also other substrate proteins Arabidopsis thaliana

General Information

EC Number General Information Comment Organism
2.7.11.24 malfunction MKKs 1 and 2 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK4KD, but do not stimulate MPK4KD to phosphorylate myelin basic protein Arabidopsis thaliana
2.7.11.24 malfunction MKKs 4 and 5 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK3KD, but do not stimulate MPK3KD to phosphorylate myelin basic protein Arabidopsis thaliana
2.7.11.24 malfunction MKKs 4 and 5 induce dual phosphorylation of the TEY motif in the activation loop of the kinase-death version of MPK6KD, but do not stimulate MPK6KD to phosphorylate myelin basic protein Arabidopsis thaliana
2.7.11.24 additional information residue Y119 is the gatekeeper in the MPK3 enzyme structure Arabidopsis thaliana
2.7.11.24 additional information residue Y124 is the gatekeeper in the MPK4 enzyme structure Arabidopsis thaliana
2.7.11.24 additional information residue Y144 is the gatekeeper in the MPK6 enzyme structure Arabidopsis thaliana