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Literature summary extracted from

  • Munoz-Clares, R.A.; Riveros-Rosas, H.; Garza-Ramos, G.; Gonzalez-Segura, L.; Mujica-Jimenez, C.; Julian-Sanchez, A.
    Exploring the evolutionary route of the acquisition of betaine aldehyde dehydrogenase activity by plant ALDH10 enzymes: implications for the synthesis of the osmoprotectant glycine betaine (2014), BMC Plant Biol., 14, 149.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.8 A441C the mutant exhibited almost wild type activity with betaine aldehyde Spinacia oleracea
1.2.1.8 A441F the mutant shows no activity with betaine aldehyde Spinacia oleracea
1.2.1.8 A441I the mutant shows no activity with betaine aldehyde Spinacia oleracea
1.2.1.8 A441S the mutant exhibits slightly reduced activity with betaine aldehyde Spinacia oleracea
1.2.1.8 A441T the mutant exhibits clearly reduced activity with betaine aldehyde Spinacia oleracea
1.2.1.8 A441V the mutant shows no activity with betaine aldehyde Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.8 0.0028
-
 NAD+ mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.0064
-
 NAD+ mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.014
-
 NAD+ mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.018
-
 NAD+ mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.022
-
 NAD+ wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.024
-
 NAD+ mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.029
-
 NAD+ mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.09
-
 Betaine aldehyde mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.098
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.119
-
 Betaine aldehyde mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.18
-
 Betaine aldehyde mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.512
-
 Betaine aldehyde mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.605
-
 Betaine aldehyde mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 1.791
-
 Betaine aldehyde mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O Spinacia oleracea
-
 betaine + NADH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.8 Spinacia oleracea P17202
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.8 betaine aldehyde + NAD+ + H2O
-
 Spinacia oleracea betaine + NADH + 2 H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.8 ALD10
-
 Spinacia oleracea
1.2.1.8 BADH
-
 Spinacia oleracea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.8 0.29
-
 Betaine aldehyde mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.39
-
 NAD+ mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.51
-
 NAD+ mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.54
-
 Betaine aldehyde mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.68
-
 NAD+ mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.74
-
 Betaine aldehyde mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 2.2
-
 NAD+ mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 2.39
-
 NAD+ mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 2.64
-
 Betaine aldehyde mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 2.99
-
 Betaine aldehyde mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 3.27
-
 NAD+ mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 3.29
-
 Betaine aldehyde mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 3.36
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 4.25
-
 NAD+ wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.8 NAD+
-
 Spinacia oleracea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.8 0.41
-
 Betaine aldehyde mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 0.48
-
 Betaine aldehyde mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 1.1
-
 Betaine aldehyde mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 15
-
 Betaine aldehyde mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 22
-
 NAD+ mutant enzyme A441F, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 28
-
 Betaine aldehyde mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 33
-
 Betaine aldehyde mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 35
-
 Betaine aldehyde wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 80
-
 NAD+ mutant enzyme A441V, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 100
-
 NAD+ mutant enzyme A441T, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 114
-
 NAD+ mutant enzyme A441S, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 179
-
 NAD+ mutant enzyme A441C, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 195
-
 NAD+ wild type enzyme, at pH 8.0 and 30°C Spinacia oleracea
1.2.1.8 243
-
 NAD+ mutant enzyme A441I, at pH 8.0 and 30°C Spinacia oleracea