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Literature summary extracted from

  • Frommhagen, M.; Sforza, S.; Westphal, A.; Visser, J.; Hinz, S.; Koetsier, M.; Van Berkel, W.; Gruppen, H.; Kabel, M.
    Discovery of the combined oxidative cleavage of plant xylan and cellulose by a new fungal polysaccharide monooxygenase (2015), Biotechnol. Biofuels, 8, 101.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.54 expression in protease-free Thielavia heterothallica Thermothelomyces thermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.54 modeling of structure. The divalent metal ion in the active site is coordinated by the three amino acids, His1, His68 and Tyr153. LPMO9A comprises two disulfide bridges, Cys126-Cys208 and Cys38-Cys156 Thermothelomyces thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.54 23000
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-
 Thermothelomyces thermophilus

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Thermothelomyces thermophilus A0A0H4K9X4
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 cellulose + ascorbate + O2 substrate regenerated amorphous cellulose Thermothelomyces thermophilus C1/C4-oxidized cellooligosaccharides + cellooligosaccharides + dehydroascorbate + H2O enzyme cleaves beta-(1->4)-glucosyl bonds in cellulose under formation of oxidized gluco-oligosaccharides. Both C1 and C4 oxidized gluco-oligosaccharides and non-oxidized gluco-oligosaccharides are formed ?
1.14.99.54 xylan + dopamine + O2
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 Thermothelomyces thermophilus C1/C4-oxidized xylooligosaccharides + 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O enzyme cleaves beta-(1->4)-xylosyl bonds in xylan under formation of oxidized xylo-oligosaccharides ?

Subunits

EC Number Subunits Comment Organism
1.14.99.54 ? x * 23000, SDS-PAGE, x * 22755, calculated, x * 22765, mass specrometry Thermothelomyces thermophilus

Synonyms

EC Number Synonyms Comment Organism
1.14.99.54 LPMO9A
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 Thermothelomyces thermophilus

General Information

EC Number General Information Comment Organism
1.14.99.54 physiological function LPMO9A shows a strong synergistic effect with endoglucanase I with a 16fold higher release of detected oligosaccharides, compared to the oligosaccharides release of LPMO9A and endoglucanase I alone Thermothelomyces thermophilus