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Literature summary extracted from

  • Wu, X.; Xu, L.; Yan, M.
    A new NAD(+)-dependent glyceraldehyde dehydrogenase obtained by rational design of l-lactaldehyde dehydrogenase from Escherichia coli (2016), Biosci. Biotechnol. Biochem., 80, 2306-2310.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.22 N286E engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde Escherichia coli
1.2.1.22 N286H engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde Escherichia coli
1.2.1.22 N286T engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde. Compared to L-lactaldehyde, N286T has a one-third lower Km value to glyceraldehyde. Escherichia coli
1.2.1.89 N286E engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde Escherichia coli
1.2.1.89 N286H engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde Escherichia coli
1.2.1.89 N286T engineering of L-lactaldehyde dehydrogenase. Contrary to wild-type, mutant is able to oxidate glyceraldehyde. Compared to L-lactaldehyde, N286T has a one-third lower Km value to glyceraldehyde. Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.22 1.27
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 5.8
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 7.5
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 14.5
-
 (S)-lactaldehyde wild-type, pH 7.0, 37°C Escherichia coli
1.2.1.89 1.14
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 1.97
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 2.2
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.22 Escherichia coli P25553
-
-
1.2.1.89 Escherichia coli P25553 cf. EC 1.2.1.22
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.22 0.74
-
 wild-type, pH 7.0, 37°C Escherichia coli
1.2.1.22 1.18
-
 mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 1.78
-
 mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 2.18
-
 mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 0.08
-
 mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 0.13
-
 mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 1.1
-
 mutant N286H, pH 7.0, 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.22 (S)-lactaldehyde + NAD+ + H2O
-
 Escherichia coli (S)-lactate + NADH + 2 H+
-
 ?
1.2.1.89 D-glyceraldehyde + NAD+ + H2O activity of mutants N286E, N286H, N286T. No substrate for wild-type Escherichia coli D-glycerate + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.22 AldA
-
 Escherichia coli
1.2.1.89 AldA
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.22 55
-
 1 h, 70% residual activity for mutant N286T Escherichia coli
1.2.1.89 55
-
 1 h, 70% residual activity for mutant N286T Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.22 761.5
-
 (S)-lactaldehyde wild-type, pH 7.0, 37°C Escherichia coli
1.2.1.22 1026
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 1545
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 1895
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 69.6
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 113.1
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 957
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.22 NAD+
-
 Escherichia coli
1.2.1.89 NAD+ mutants N286E, N286H, N286T, but not wild-type show activtiy with NAD+ Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.22 52.5
-
 (S)-lactaldehyde wild-type, pH 7.0, 37°C Escherichia coli
1.2.1.22 206.1
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 326.8
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.22 808.1
-
 (S)-lactaldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 31.64
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 99.2
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli
1.2.1.89 485.8
-
 D-glyceraldehyde mutant N286H, pH 7.0, 37°C Escherichia coli