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Literature summary extracted from

  • Chen, X.; Yang, C.; Wang, P.; Zhang, X.; Bao, B.; Li, D.; Shi, R.
    Stereoselective biotransformation of racemic mandelic acid using immobilized laccase and (S)-mandelate dehydrogenase (2017), Bioresour. Bioproc., 4, 2-2.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.99.31 0.27
-
 (S)-Mandelate chitosan-immobilized enzyme, at pH 3.4 and 45°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.99.31 (S)-mandelate + FMN Escherichia coli
-
 benzoylformic acid + FMNH2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.99.31 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.99.31 Toyopearl DEAE-650 M column chromatography Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.99.31 (S)-mandelate + FMN
-
 Escherichia coli benzoylformic acid + FMNH2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.99.31 SMDH
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.99.31 45
-
 chitosan-immobilized enzyme Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.99.31 3.4
-
 chitosan-immobilized enzyme Escherichia coli