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Literature summary extracted from

  • Takenaka, M.; Yoon, K.S.; Matsumoto, T.; Ogo, S.
    Acetyl-CoA production by encapsulated pyruvate ferredoxin oxidoreductase in alginate hydrogels (2017), Biores. Technol., 227, 279-285.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.2.7.1 the operational stability of the enzyme in alginate hydrogels retains over 68% initial activity after 10 repeated cycles Citrobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.7.1 0.028
-
 CoA free enzyme, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 0.12
-
 pyruvate free enzyme, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 0.37
-
 pyruvate enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 0.54
-
 CoA enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C Citrobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.1 Mg2+ 2 mM used in assay conditions Citrobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.7.1 120000
-
 2 * 120000, SDS-PAGE Citrobacter sp.
1.2.7.1 240000
-
 gel filtration Citrobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.1 Citrobacter sp.
-
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.2.7.1 the enzyme shows high O2-sensitivity Citrobacter sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.7.1 Q-Sepharose column chromatography, phenyl Sepharose column chromatography, and Superdex 200 gel filtration Citrobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.1 additional information the enzyme is incapable of using NAD+ and NADP+ as electron acceptors Citrobacter sp. ?
-
 ?
1.2.7.1 pyruvate + CoA + oxidized methyl viologen
-
 Citrobacter sp. acetyl-CoA + CO2 + reduced methyl viologen + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.7.1 homodimer 2 * 120000, SDS-PAGE Citrobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.2.7.1 PFOR
-
 Citrobacter sp.
1.2.7.1 pyruvate ferredoxin oxidoreductase
-
 Citrobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.7.1 30
-
-
 Citrobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.7.1 30 50 the enzyme retains more than 60% of the initial activity 30-50°C, even after 30 min incubation Citrobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.7.1 35
-
 pyruvate enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 39
-
 CoA enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 94
-
 pyruvate free enzyme, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 109
-
 CoA free enzyme, at pH 7.0 and 30°C Citrobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.1 7
-
-
 Citrobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.7.1 6 8 the enzyme retains more than 70% of the initial activity at pH 6.0-8.0, even after 30 min incubation Citrobacter sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.7.1 thiamine diphosphate
-
 Citrobacter sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.7.1 72
-
 CoA enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 95
-
 pyruvate enzyme immobilized in alginate hydrogel, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 780
-
 pyruvate free enzyme, at pH 7.0 and 30°C Citrobacter sp.
1.2.7.1 3900
-
 CoA free enzyme, at pH 7.0 and 30°C Citrobacter sp.