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Literature summary extracted from

  • Tiwari, M.K.; Singh, R.K.; Gao, H.; Kim, T.; Chang, S.; Kim, H.S.; Lee, J.K.
    pH-rate profiles of L-arabinitol 4-dehydrogenase from Hypocrea jecorina and its application in L-xylulose production (2014), Bioorg. Med. Chem. Lett., 24, 173-176.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.1.1.12 synthesis rare L-sugar L-xylulose is produced by the enzymatic oxidation of arabinitol to give a yield of approximately 86% Trichoderma reesei

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.12 gene lad1, overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.12 H91A site-directed mutagenesis, catalytically inactive mutant Trichoderma reesei
1.1.1.12 additional information all tested wild-type and mutant enzymes have a similar secondary structure and the global folding of mutant and wild-type HjLAD is similar Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.12 0.2
-
 NAD+ pH 9.5, 25°C Trichoderma reesei
1.1.1.12 0.2
-
 NAD+ pH 7.0, 25°C Trichoderma reesei
1.1.1.12 0.5
-
 NAD+ pH 8.0, 25°C Trichoderma reesei
1.1.1.12 14.5
-
 L-arabinitol pH 9.5, 25°C Trichoderma reesei
1.1.1.12 17.2
-
 L-arabinitol pH 8.0, 25°C Trichoderma reesei
1.1.1.12 18.2
-
 L-arabinitol pH 7.0, 25°C Trichoderma reesei

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.12 Zn2+ the Cys66, His91, Glu92, and Glu176 residues are involved in coordination of catalytic Zn2+ along with a water molecule Trichoderma reesei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.12 40000
-
 x * 40000, SDS-PAGE Trichoderma reesei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.12 L-arabinitol + NAD+ Trichoderma reesei
-
 L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+ Trichoderma reesei YSM 768
-
 L-xylulose + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.12 Trichoderma reesei Q96V44 i.e Trichoderma reesei
-
1.1.1.12 Trichoderma reesei YSM 768 Q96V44 i.e Trichoderma reesei
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.12 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Trichoderma reesei

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.12 24
-
 purified recombinant His-tagged enzyme, pH 7.0, 25°C Trichoderma reesei
1.1.1.12 44
-
 purified recombinant His-tagged enzyme, pH 8.0, 25°C Trichoderma reesei
1.1.1.12 105
-
 purified recombinant His-tagged enzyme, pH 9.5, 25°C Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.12 L-arabinitol + NAD+
-
 Trichoderma reesei L-xylulose + NADH + H+
-
 ?
1.1.1.12 L-arabinitol + NAD+
-
 Trichoderma reesei YSM 768 L-xylulose + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.12 ? x * 40000, SDS-PAGE Trichoderma reesei
1.1.1.12 More homology modeling and docking of L-arabinitol in the substrate-binding pocket of HjLAD, molecular dynamics, overview Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
1.1.1.12 LAD
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.12 25
-
 assay at Trichoderma reesei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.12 16
-
 L-arabinitol pH 7.0, 25°C Trichoderma reesei
1.1.1.12 29.3
-
 L-arabinitol pH 8.0, 25°C Trichoderma reesei
1.1.1.12 70
-
 L-arabinitol pH 9.5, 25°C Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.12 9.5
-
-
 Trichoderma reesei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.12 7 9.5 pH-profile, overview Trichoderma reesei

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.12 NAD+
-
 Trichoderma reesei

General Information

EC Number General Information Comment Organism
1.1.1.12 additional information homology modeling and docking of L-arabinitol in the substrate-binding pocket of HjLAD suggesting routes of hydride transfer, where the key amino acid residues comprise the core region, molecular dynamics, overview Trichoderma reesei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.12 0.88
-
 L-arabinitol pH 7.0, 25°C Trichoderma reesei
1.1.1.12 1.7
-
 L-arabinitol pH 8.0, 25°C Trichoderma reesei
1.1.1.12 4.83
-
 L-arabinitol pH 9.5, 25°C Trichoderma reesei