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Literature summary extracted from

  • Bontemps-Gallo, S.; Cogez, V.; Robbe-Masselot, C.; Quintard, K.; Dondeyne, J.; Madec, E.; Lacroix, J.M.
    Biosynthesis of osmoregulated periplasmic glucans in Escherichia coli: the phosphoethanolamine transferase is encoded by opgE (2013), BioMed Res. Int., 2013, 371429.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.8.43 arbutin arbutin is also a substrate for phosphoethanolamine transferase stimulating its activity, increasing phosphatidylethanolamine turnover leading to accumulation of diacylglycerol toxic for bacteria Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.8.43 genetic screening identifies gene opgE, DNA and amino acid sequence determination and analysis, genetic organization of opg genes in Escherichia coli, overview. The gene is transformed into competent Escherichia coli cells to give pNF752 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.7.8.43 additional information construction of the opgE::cml mutant, and of the opgE::Tn5 mutant by random transposon mutagenesis with Tn5 Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.43 inner membrane the enzyme is located on the periplasmic side of the inner membrane Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.8.43 additional information Escherichia coli the phosphoethanolamine cycle, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.8.43 Escherichia coli P75785
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.43 diacylphosphatidylethanolamine + arbutin arbutin is a substrate for the phosphoethanolamine transferase Escherichia coli diacylglycerol + ?
-
 ?
2.7.8.43 additional information the phosphoethanolamine cycle, overview Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.8.43 opgE
-
 Escherichia coli
2.7.8.43 phosphoethanolamine transferase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.8.43 37
-
 assay at Escherichia coli

General Information

EC Number General Information Comment Organism
2.7.8.43 physiological function the enzyme is required for substitution of osmoregulated periplasmic glucans by phosphoethanolamine Escherichia coli