EC Number | Application | Comment | Organism |
---|---|---|---|
1.7.1.16 | analysis | immobilization of enzyme on polyethyleneimine-mediated silica formation, enzyme activity is significantly more stable than NbzA in solution. A microfluidic microreactor is suitable for continuous operation using nitrobenzene, CB1954, and the proantibiotic nitrofurazone. The flow-through system provides a rapid and reproducible screening method for determining the NbzA-catalyzed activation of prodrugs and proantibiotics | Pseudomonas oleovorans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.7.1.16 | expression in Escherichia coli | Pseudomonas oleovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.7.1.16 | 0.002 | - |
nitrobenzene | imobilized enzyme, 25°C, pH not specified in the publication | Pseudomonas oleovorans | |
1.7.1.16 | 0.0023 | - |
nitrobenzene | 25°C, pH not specified in the publication | Pseudomonas oleovorans | |
1.7.1.16 | 0.0117 | - |
5-(aziridin-1-yl)-2,4-dinitrobenzamide | 25°C, pH not specified in the publication | Pseudomonas oleovorans | |
1.7.1.16 | 0.0337 | - |
5-(aziridin-1-yl)-2,4-dinitrobenzamide | imobilized enzyme, 25°C, pH not specified in the publication | Pseudomonas oleovorans | |
1.7.1.16 | 1.763 | - |
nitrofurazone | 25°C, pH not specified in the publication | Pseudomonas oleovorans | |
1.7.1.16 | 5.123 | - |
nitrofurazone | imobilized enzyme, 25°C, pH not specified in the publication | Pseudomonas oleovorans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.7.1.16 | Pseudomonas oleovorans | Q6DLR9 | - |
- |
1.7.1.16 | Pseudomonas oleovorans JS45 | Q6DLR9 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.7.1.16 | - |
Pseudomonas oleovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.7.1.16 | 5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans | ? + 2 NADP+ + H2O | enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide | ? | |
1.7.1.16 | 5-(aziridin-1-yl)-2,4-dinitrobenzamide + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans JS45 | ? + 2 NADP+ + H2O | enzyme activates the dinitrobenzamide cancer prodrug CB1954, i.e. 5-(aziridin-1-yl)-2,4-dinitrobenzamide | ? | |
1.7.1.16 | nitrobenzene + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans | N-hydroxyaniline + 2 NADP+ + H2O | - |
? | |
1.7.1.16 | nitrobenzene + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans JS45 | N-hydroxyaniline + 2 NADP+ + H2O | - |
? | |
1.7.1.16 | nitrofurazone + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans | ? + 2 NADP+ + H2O | enzyme activates the proantibiotic nitrofurazone | ? | |
1.7.1.16 | nitrofurazone + 2 NADPH + 2 H+ | - |
Pseudomonas oleovorans JS45 | ? + 2 NADP+ + H2O | enzyme activates the proantibiotic nitrofurazone | ? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.7.1.16 | NbzA | - |
Pseudomonas oleovorans |