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Literature summary extracted from

  • McGinnis, L.A.; Lee, H.J.; Robinson, D.N.; Evans, J.P.
    MAPK3/1 (ERK1/2) and myosin light chain kinase in mammalian eggs affect myosin-II function and regulate the metaphase II state in a calcium- and zinc-dependent manner (2015), Biol. Reprod., 92, 146.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 Calmodulin required Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 ML-7
-
 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ dependent on Mus musculus
2.7.11.18 Mg2+ dependent on Mus musculus
2.7.11.18 Zn2+ dependent on Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + [myosin light chain] Mus musculus
-
 ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain] Mus musculus CF-1
-
 ADP + [myosin light chain] phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Mus musculus Q3UIZ8
-
-
2.7.11.18 Mus musculus CF-1 Q3UIZ8
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.18 egg ovulated metaphase II eggs Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + [myosin light chain]
-
 Mus musculus ADP + [myosin light chain] phosphate
-
 ?
2.7.11.18 ATP + [myosin light chain]
-
 Mus musculus CF-1 ADP + [myosin light chain] phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 MLCK
-
 Mus musculus
2.7.11.18 myosin light chain kinase
-
 Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 ATP
-
 Mus musculus

General Information

EC Number General Information Comment Organism
2.7.11.18 malfunction in meiosis I, MLCK inhibition and other myosin perturbations impair spindle migration and first polar body emission. Inhibition of the MEK1/2-MAPK pathway with U0126 leads to reduced levels of phosphorylated myosin-regulatory light chain (pMRLC) and causes a reduction in cortical tension, effects that are mimicked by treatment with the myosin light chain kinase (MLCK) inhibitor ML-7. MAPK or MLCK inhibition induces loss of normal cortical spindle localization or parthenogenetic egg activation. This parthenogenesis is dependent on cytosolic and extracellular calcium and can be rescued by hyperloading eggs with zinc, suggesting that these effects of inhibition of MLCK or the MAPK pathway are linked with dysregulation of ion homeostasis, overview Mus musculus
2.7.11.18 metabolism MAPK3/1 (ERK1/2, EC 2.7.11.24) and myosin light chain kinase in mammalian eggs affect myosin-II function and regulate the metaphase II state in a calcium- and zinc-dependent manner. Vertebrate eggs are arrested at metaphase of meiosis II, a state classically known as cytostatic factor arrest. Maintenance of this arrest until the time of fertilization and then fertilizationinduced exit from metaphase II are crucial for reproductive success, overview Mus musculus
2.7.11.18 physiological function MLCK phosphorylates myosin regulatory light chain (MRLC, also known as MYL9), which enables myosin-II organization into bipolar thick filaments Mus musculus