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Literature summary extracted from
- Isolation and characterization of isopiperitenol dehydrogenase from piperitenone-type Perilla (2014), Biol. Pharm. Bull., 37, 847-852.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.1.223 | gene PK-ISPD1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F' | Perilla frutescens var. hirtella |
| 1.1.1.223 | gene PT-ISPD2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F' | Perilla frutescens |
| 1.1.1.223 | gene PT-ISPD2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain TOP10F' | Perilla frutescens var. hirtella |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | Perilla frutescens var. hirtella | - |
(-)-isopiperitenone + NADH + H+ | - |
r |  |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | Perilla frutescens | - |
(-)-isopiperitenone + NADH + H+ | - |
ir | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | Perilla frutescens var. hirtella | - |
(-)-isopiperitenone + NADH + H+ | - |
ir | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | Perilla frutescens 5598 | - |
(-)-isopiperitenone + NADH + H+ | - |
ir | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | Perilla frutescens var. hirtella 5712 | - |
(-)-isopiperitenone + NADH + H+ | - |
ir | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | Perilla frutescens var. hirtella 5712 | - |
(-)-isopiperitenone + NADH + H+ | - |
r | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.1.223 | Perilla frutescens | W5VJJ6 | PT-type | - |
| 1.1.1.223 | Perilla frutescens 5598 | W5VJJ6 | PT-type | - |
| 1.1.1.223 | Perilla frutescens var. hirtella | W5VJM4 | - |
- |
| 1.1.1.223 | Perilla frutescens var. hirtella | W5VJT8 | PK-type, not found in not found in PT-type strains nos. 5598 and 5601 | - |
| 1.1.1.223 | Perilla frutescens var. hirtella 5712 | W5VJM4 | - |
- |
| 1.1.1.223 | Perilla frutescens var. hirtella 5712 | W5VJT8 | PK-type, not found in not found in PT-type strains nos. 5598 and 5601 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.1.223 | recombinant His-tagged enzyme from Escherichia coli strain TOP10F' | Perilla frutescens var. hirtella |
| 1.1.1.223 | recombinant His-tagged enzyme from Escherichia coli strain TOP10F' | Perilla frutescens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.1.223 | leaf | young | Perilla frutescens var. hirtella | - |
| 1.1.1.223 | leaf | young | Perilla frutescens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella | (-)-isopiperitenone + NADH + H+ | - |
r | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens | (-)-isopiperitenone + NADH + H+ | - |
ir | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella | (-)-isopiperitenone + NADH + H+ | - |
ir | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens 5598 | (-)-isopiperitenone + NADH + H+ | - |
ir | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella 5712 | (-)-isopiperitenone + NADH + H+ | - |
ir | |
| 1.1.1.223 | (-)-cis-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella 5712 | (-)-isopiperitenone + NADH + H+ | - |
r | |
| 1.1.1.223 | (-)-trans-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella | (-)-isopiperitenone + NADH + H+ | - |
r | |
| 1.1.1.223 | (-)-trans-isopiperitenol + NAD+ | - |
Perilla frutescens var. hirtella 5712 | (-)-isopiperitenone + NADH + H+ | - |
r | |
| 1.1.1.223 | additional information | ISPD1 has broader substrate specificity than ISPD2 | Perilla frutescens var. hirtella | ? | - |
? | |
| 1.1.1.223 | additional information | ISPD2 has a greater substrate specificity compared to ISPD1 | Perilla frutescens | ? | - |
? | |
| 1.1.1.223 | additional information | ISPD2 has a greater substrate specificity compared to ISPD1 | Perilla frutescens var. hirtella | ? | - |
? | |
| 1.1.1.223 | additional information | ISPD2 has a greater substrate specificity compared to ISPD1 | Perilla frutescens 5598 | ? | - |
? | |
| 1.1.1.223 | additional information | ISPD2 has a greater substrate specificity compared to ISPD1 | Perilla frutescens var. hirtella 5712 | ? | - |
? | |
| 1.1.1.223 | additional information | ISPD1 has broader substrate specificity than ISPD2 | Perilla frutescens var. hirtella 5712 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.1.223 | isopiperitenol dehydrogenase 1 | - |
Perilla frutescens var. hirtella |
| 1.1.1.223 | isopiperitenol dehydrogenase 2 | - |
Perilla frutescens |
| 1.1.1.223 | isopiperitenol dehydrogenase 2 | - |
Perilla frutescens var. hirtella |
| 1.1.1.223 | ISPD1 | - |
Perilla frutescens var. hirtella |
| 1.1.1.223 | ISPD2 | - |
Perilla frutescens |
| 1.1.1.223 | ISPD2 | - |
Perilla frutescens var. hirtella |
| 1.1.1.223 | PK-ISPD1 | - |
Perilla frutescens var. hirtella |
| 1.1.1.223 | PK-ISPD2 | - |
Perilla frutescens var. hirtella |
| 1.1.1.223 | PT-ISPD2 | - |
Perilla frutescens |
| 1.1.1.223 | PT-ISPD2 | - |
Perilla frutescens var. hirtella |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.1.223 | NAD+ | - |
Perilla frutescens var. hirtella | |
| 1.1.1.223 | NAD+ | - |
Perilla frutescens | |
| 1.1.1.223 | NADH | - |
Perilla frutescens var. hirtella | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.1.223 | metabolism | the enzyme is involved in the biosynthetic pathways of oil constituents (PA-type, PT-type, and PK-type) in Perilla, putative pathways, overview | Perilla frutescens var. hirtella |
| 1.1.1.223 | metabolism | the enzyme is involved in the biosynthetic pathways of oil constituents (PA-type, PT-type, and PK-type) in Perilla, putative pathways, overview. (-)-cis-Isopiperitenol is an intermediate for the piperitenone biosynthesis of perilla | Perilla frutescens |
| 1.1.1.223 | metabolism | the enzyme is involved in the biosynthetic pathways of oil constituents (PA-type, PT-type, and PK-type) in Perilla, putative pathways, overview. (-)-cis-Isopiperitenol is an intermediate for the piperitenone biosynthesis of perilla | Perilla frutescens var. hirtella |
| 1.1.1.223 | physiological function | isozyme ISPD1 is less likely to be involved in oxidation of isopiperitenol | Perilla frutescens var. hirtella |
| 1.1.1.223 | physiological function | isozyme ISPD2 is likely to be involved in oxidation of isopiperitenol | Perilla frutescens |
| 1.1.1.223 | physiological function | isozyme ISPD2 is likely to be involved in oxidation of isopiperitenol | Perilla frutescens var. hirtella |
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