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Literature summary extracted from

  • Guo, Q.; Gakhar, L.; Wickersham, K.; Francis, K.; Vardi-Kilshtain, A.; Major, D.T.; Cheatum, C.M.; Kohen, A.
    Structural and kinetic studies of formate dehydrogenase from Candida boidinii (2016), Biochemistry, 55, 2760-2771.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.9 expressed in Escherichia coli BL21(DE3) pLysS cells [Candida] boidinii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.17.1.9 hanging drop vapor diffusion method [Candida] boidinii

Protein Variants

EC Number Protein Variants Comment Organism
1.17.1.9 K328V the mutant shows increased catalytic efficiency for NAD+ compared to the wild type enzyme [Candida] boidinii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 azide nanomolar inhibitor [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.025
-
 NAD+ mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 0.0303
-
 NAD+ recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 4
-
 formate recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 5
-
 formate mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 [Candida] boidinii O13437
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.17.1.9 Blue Sepharose 6 column chromatography and Superdex 200 gel filtration [Candida] boidinii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 FDH
-
 [Candida] boidinii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 5.6
-
 NAD+ recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 5.6
-
 formate recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 6.8
-
 NAD+ mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 6.8
-
 formate mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 [Candida] boidinii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17.1.9 1.4
-
 formate recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 1.4
-
 formate mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 180
-
 NAD+ recombinant enzyme, at pH 7.5 and 25°C [Candida] boidinii
1.17.1.9 270
-
 NAD+ mutant enzyme K328V, at pH 7.5 and 25°C [Candida] boidinii