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Literature summary extracted from

  • Li, L.; Ye, C.; Zhao, R.; Li, X.; Liu, W.Z.; Wu, F.; Yan, J.; Jiang, Y.Q.; Yang, B.
    Mitogen-activated protein kinase kinase kinase (MAPKKK) 4 from rapeseed (Brassica napus L.) is a novel member inducing ROS accumulation and cell death (2015), Biochem. Biophys. Res. Commun., 467, 792-797.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.25 gene MAPKKK4.1, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, quantitative RT-PCR expression analysis, recombinant expression in transgenic Nicotiana benthamiana leaves under the driving of CaMV35S promoter, recombinant coexpression with substrate proteins MKK3 and MKK6 in Saccharomyces cerevisiae strain AH109 cells Brassica napus
2.7.11.25 gene MAPKKK4.2, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic tree, quantitative RT-PCR expression analysis, recombinant expression in transgenic Nicotiana benthamiana leaves under the driving of CaMV35S promoter, recombinant coexpression with substrate proteins MKK3 and MKK6 in Saccharomyces cerevisiae strain AH109 cells Brassica napus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.25 Mg2+ required Brassica napus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.25 ATP + MKK3 Brassica napus substrate Brassica napus MAPK kinase 3 ADP + phosphorylated MKK3
-
 ?
2.7.11.25 ATP + MKK6 Brassica napus substrate Brassica napus MAPK kinase 6 ADP + phosphorylated MKK6
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.25 Brassica napus A0A0U1XFB1 gene MAPKKK4.2
-
2.7.11.25 Brassica napus A0A0U1XFE3 gene MAPKKK4.1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.25 ATP + MKK3 substrate Brassica napus MAPK kinase 3 Brassica napus ADP + phosphorylated MKK3
-
 ?
2.7.11.25 ATP + MKK6 substrate Brassica napus MAPK kinase 6 Brassica napus ADP + phosphorylated MKK6
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.25 BnaMAPKKK4.2
-
 Brassica napus
2.7.11.25 MAPKKK4
-
 Brassica napus
2.7.11.25 mitogen-activated protein kinase kinase kinase 4
-
 Brassica napus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.25 ATP
-
 Brassica napus

General Information

EC Number General Information Comment Organism
2.7.11.25 metabolism MAPKK kinase family enzymes play important roles in plant growth, development and immune responses. The two downstream kinases MKK3 and MKK6 act in the BnaMAPKKK4 signaling pathway Brassica napus
2.7.11.25 physiological function expression of MAPKKK4 elicits reactive oxgen species, ROS, i.e. H2O2 accumulation and cell death. Upregulation of enzyme MAPKKK4 leads to increased expression of ROS accumulation, cell death, and defense response related genes Brassica napus