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Literature summary extracted from

  • Endo, S.; Matsunaga, T.; Ikari, A.; El-Kabbani, O.; Hara, A.; Kitade, Y.
    Identification of a determinant for strict NADP(H)-specificity and high sensitivity to mixed-type steroid inhibitor of rabbit aldo-keto reductase 1C33 by site-directed mutagenesis (2015), Arch. Biochem. Biophys., 569, 19-25.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.357 F217S mutation converts AKR1C33 into a dually coenzyme-specific form and abolishes the activity towards 3-keto-5beta-cholestanes that are substrates specific to AKR1C33, and markedly decreases the sensitivity to 4-pregnenes Oryctolagus cuniculus
1.1.1.357 N272T mutation does not influence cofactor specificity Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.357 NADPH inhibition pattern is competitive Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.357 0.0001
-
 5alpha-androstan-17-beta-ol-3-one mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0002
-
 NADP+ mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0002
-
 5alpha-pregnan-20alpha-ol-3-one mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0003
-
 5alpha-pregnan-20alpha-ol-3-one wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0004
-
 NADPH mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0005
-
 5beta-pregnane-3,20-dione mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0006
-
 5beta-androstan-3,17-dione mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0006
-
 5beta-Androstan-3alpha-ol-17-one mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0009
-
 Dehydrolithocholic acid mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0021
-
 5beta-Androstan-3alpha-ol-17-one wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0023
-
 5beta-pregnane-3,20-dione wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.003
-
 5beta-androstan-3,17-dione wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.003
-
 5alpha-androstan-17-beta-ol-3-one wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.004
-
 Dehydrolithocholic acid wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0084
-
 NADPH wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.009
-
 NADPH mutant N272T, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.017
-
 NADP+ wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.017
-
 NADP+ mutant N272T, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.033
-
 NADH mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.081
-
 NAD+ mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.97
-
 NAD+ wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 1
-
 NAD+ mutant N272T, pH 7.4, 25°C Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.357 Oryctolagus cuniculus T2HVB4
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.357 5alpha-androstan-17-beta-ol-3-one + NADH + H+ NADH is not a substrate for wild-type Oryctolagus cuniculus 5alpha-androstane-3alpha,17beta-diol + NAD+
-
 r
1.1.1.357 5alpha-androstan-17-beta-ol-3-one + NADPH + H+
-
 Oryctolagus cuniculus 5alpha-androstane-3alpha,17beta-diol + NADP+
-
 r
1.1.1.357 5alpha-androstane-3alpha,17beta-diol + NAD+
-
 Oryctolagus cuniculus 5alpha-androstan-17-beta-ol-3-one + NADH + H+
-
 r
1.1.1.357 5alpha-androstane-3alpha,17beta-diol + NADP+
-
 Oryctolagus cuniculus 5alpha-androstan-17-beta-ol-3-one + NADPH + H+
-
 r
1.1.1.357 5alpha-pregnan-20alpha-ol-3-one + NADP+
-
 Oryctolagus cuniculus 5alpha-pregnane-3,20-dione + NADPH + H+
-
 r
1.1.1.357 5beta-androstan-3,17-dione + NADPH + H+
-
 Oryctolagus cuniculus 5beta-androstan-3alpha-ol-17-one + NADP+
-
 r
1.1.1.357 5beta-androstan-3alpha-ol-17-one + NADP+
-
 Oryctolagus cuniculus 5beta-androstan-3,17-dione + NADPH + H+
-
 r
1.1.1.357 5beta-pregnane-3,20-dione + NADPH + H+
-
 Oryctolagus cuniculus 5beta-pregnan-20alpha-ol-3-one + NADP+
-
 r
1.1.1.357 dehydrolithocholic acid + NADPH + H+
-
 Oryctolagus cuniculus ?
-
 r
1.1.1.357 additional information enzyme is strictly NADPH-specific Oryctolagus cuniculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.357 AKR1C33
-
 Oryctolagus cuniculus
1.1.1.357 Naloxone reductase 2
-
 Oryctolagus cuniculus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.357 0.0025
-
 5alpha-pregnan-20alpha-ol-3-one mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.004
-
 5beta-Androstan-3alpha-ol-17-one mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.004
-
 5alpha-androstan-17-beta-ol-3-one mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.006
-
 NADP+ mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.006
-
 Dehydrolithocholic acid mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.007
-
 5beta-pregnane-3,20-dione mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.009
-
 5beta-androstan-3,17-dione mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.01
-
 NADPH mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.037
-
 Dehydrolithocholic acid wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.05
-
 5alpha-pregnan-20alpha-ol-3-one wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.053
-
 5beta-pregnane-3,20-dione wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.077
-
 NADPH wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.1
-
 NADPH mutant N272T, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.113
-
 5beta-Androstan-3alpha-ol-17-one wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.14
-
 5beta-androstan-3,17-dione wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.367
-
 5alpha-androstan-17-beta-ol-3-one wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.72
-
 NADP+ mutant N272T, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.85
-
 NADP+ wild-type, pH 7.4, 25°C Oryctolagus cuniculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.357 0.000015
-
 NADPH mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 0.0046
-
 NADPH wild-type, pH 7.4, 25°C Oryctolagus cuniculus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.357 9
-
 NADPH wild-type, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 11
-
 NADPH mutant N272T, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 27
-
 NADPH mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 28
-
 NADP+ mutant F217S, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 42
-
 NADP+ mutant N272T, pH 7.4, 25°C Oryctolagus cuniculus
1.1.1.357 50
-
 NADP+ wild-type, pH 7.4, 25°C Oryctolagus cuniculus