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Literature summary extracted from

  • Kojima, Y.; Várnai, A.; Ishida, T.; Sunagawa, N.; Petrovic, D.M.; Igarashi, K., Jellison, J.; Goodell, B.; Alfredsen, G.; Westereng, B.; Eijsink, V.G.; Yoshida, M.
    A lytic polysaccharide monooxygenase with broad xyloglucan specificity from the brown-rot fungus Gloeophyllum trabeum and its action on cellulose-xyloglucan complexes (2016), Appl. Environ. Microbiol., 82, 6557-6572.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.B10 expression of splice variant LPMO9A-2 in Pichia pastoris Gloeophyllum trabeum
1.14.99.B10 expression of splice variant LpmoA-2 in Pichia pastoris Gloeophyllum trabeum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.99.B10 additional information hemicellulose coating has an inhibitory effect on cellulolytic LPMO activity Gloeophyllum trabeum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.99.B10 25100
-
-
 Gloeophyllum trabeum
1.14.99.B10 25800
-
-
 Gloeophyllum trabeum
1.14.99.B10 33100
-
 x * 33100, calculated Gloeophyllum trabeum
1.14.99.B10 36000
-
-
 Gloeophyllum trabeum
1.14.99.B10 60000
-
-
 Gloeophyllum trabeum
1.14.99.B10 60000
-
 gel filtration Gloeophyllum trabeum

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.B10 Gloeophyllum trabeum
-
-
-
1.14.99.B10 Gloeophyllum trabeum A0A1C9ZMC3 isoform LPMO9C
-
1.14.99.B10 Gloeophyllum trabeum A0A1C9ZMC5 splice variant LPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function
-
1.14.99.B10 Gloeophyllum trabeum A0A1C9ZP88 splice variant LPMO9A-1
-
1.14.99.B10 Gloeophyllum trabeum A0A1C9ZUN9 isoform LPMO9D
-
1.14.99.B10 Gloeophyllum trabeum NBRC 6430 A0A1C9ZMC3 isoform LPMO9C
-
1.14.99.B10 Gloeophyllum trabeum NBRC 6430 A0A1C9ZMC5 splice variant LPMO9A-2, which contains a C-terminal domain comprising approximately 55 residues without a predicted function
-
1.14.99.B10 Gloeophyllum trabeum NBRC 6430 A0A1C9ZP88 splice variant LPMO9A-1
-
1.14.99.B10 Gloeophyllum trabeum NBRC 6430 A0A1C9ZUN9 isoform LPMO9D
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.14.99.B10 glycoprotein treatment with EndoH leads to a decrease in molecular mass from 60 kDA to 57 kDa Gloeophyllum trabeum
1.14.99.B10 glycoprotein treatment with EndoH leads to a decrease in molecular mass from 60000 Da to 57000 Da Gloeophyllum trabeum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.B10 carboxymethyl cellulose + dithiothreitol + O2
-
 Gloeophyllum trabeum carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O
-
 ?
1.14.99.B10 carboxymethyl cellulose + dithiothreitol + O2 reaction is slower with electron donor dithiothreitol than with ascorbate. Reaction is 5- to 10fold slower with carboxymethyl cellulose than with xyloglucan Gloeophyllum trabeum carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O
-
 ?
1.14.99.B10 carboxymethyl cellulose + dithiothreitol + O2 reaction is slower with electron donor dithiothreitol than with ascorbate. Reaction is 5- to 10fold slower with carboxymethyl cellulose than with xyloglucan Gloeophyllum trabeum NBRC 6430 carboxymethyl cellooligosaccharides + dithiothreitol disulfide + H2O
-
 ?
1.14.99.B10 cellulose + dithiothreitol + O2
-
 Gloeophyllum trabeum oligosaccharides + dithiothreitol disulfide + H2O
-
 ?
1.14.99.B10 glucomannan + ascorbate + O2
-
 Gloeophyllum trabeum cellooligosaccharides + dehydroascorbate + H2O
-
 ?
1.14.99.B10 glucomannan + ascorbate + O2
-
 Gloeophyllum trabeum NBRC 6430 cellooligosaccharides + dehydroascorbate + H2O
-
 ?
1.14.99.B10 glucomannan + dithiothreitol + O2
-
 Gloeophyllum trabeum cellooligosaccharides + dithiothreitol disulfide + H2O minor activity on glucomannan ?
1.14.99.B10 additional information inactive on shorter cellooligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan, ivory nut mannan, wheat arabinoxylan, oat spelt xylan, beech wood xylan, and birchwood xylan or mixed linked beta-glucan. Presence of an electron donor is required Gloeophyllum trabeum ?
-
 ?
1.14.99.B10 additional information isoform LpmoA-2 has broad specificity, cleaving at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Inactive on shorter cello-oligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan Gloeophyllum trabeum ?
-
 ?
1.14.99.B10 additional information inactive on shorter cellooligosaccharides, such as cellopentaose and cellohexaose, and on arabinoxylan, ivory nut mannan, wheat arabinoxylan, oat spelt xylan, beech wood xylan, and birchwood xylan or mixed linked beta-glucan. Presence of an electron donor is required Gloeophyllum trabeum NBRC 6430 ?
-
 ?
1.14.99.B10 phosphoric acid swollen cellulose + ascorbate + O2
-
 Gloeophyllum trabeum cellooligosaccharides + dehydroascorbate + H2O
-
 ?
1.14.99.B10 phosphoric acid swollen cellulose + ascorbate + O2
-
 Gloeophyllum trabeum NBRC 6430 cellooligosaccharides + dehydroascorbate + H2O
-
 ?
1.14.99.B10 xyloglucan + ascorbate + O2
-
 Gloeophyllum trabeum ? + dehydroascorbate + H2O releases a broad range of xyloglucan oligosaccharides, cleaving occurs at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Dominating products contain a four-glucan backbone (XXXG fragments with various degrees of galactosylation), plus C-1-oxidized and C-4-oxidized cellooligosaccharides ?
1.14.99.B10 xyloglucan + ascorbate + O2
-
 Gloeophyllum trabeum NBRC 6430 ? + dehydroascorbate + H2O releases a broad range of xyloglucan oligosaccharides, cleaving occurs at any position along the beta-glucan backbone of xyloglucan, regardless of substitutions. Dominating products contain a four-glucan backbone (XXXG fragments with various degrees of galactosylation), plus C-1-oxidized and C-4-oxidized cellooligosaccharides ?
1.14.99.B10 xyloglucan + dithiothreitol + O2 enzyme acts preferentially on free xyloglucan with release of a broad range of xyloglucan oligosaccharides Gloeophyllum trabeum xyloglucan oligosaccharides + dithiothreitol disulfide + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.99.B10 ? x * 33100, calculated Gloeophyllum trabeum
1.14.99.B10 ? x * 25100, calculated Gloeophyllum trabeum
1.14.99.B10 ? x * 25800, calculated Gloeophyllum trabeum
1.14.99.B10 ? x * 36000, splicing variant Lpmo9A-2, calculated Gloeophyllum trabeum
1.14.99.B10 ? x * 60000, SDS-PAGE, x * 36000, calculated Gloeophyllum trabeum

Synonyms

EC Number Synonyms Comment Organism
1.14.99.B10 LPMO9D
-
 Gloeophyllum trabeum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.99.B10 Gloeophyllum trabeum calculated
-
 4.6
1.14.99.B10 Gloeophyllum trabeum splicing variant Lpmo9A-2, calculated
-
 4.6
1.14.99.B10 Gloeophyllum trabeum calculated
-
 4.7
1.14.99.B10 Gloeophyllum trabeum calculated
-
 4.9
1.14.99.B10 Gloeophyllum trabeum calculated
-
 5.8