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Literature summary extracted from

  • Kataoka, N.; Matsutani, M.; Yakushi, T.; Matsushita, K.
    Efficient production of 2,5-diketo-D-gluconate via heterologous expression of 2-ketogluconate dehydrogenase in Gluconobacter japonicus (2015), Appl. Environ. Microbiol., 81, 3552-3560.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.215 gene ga2dh Gluconobacter japonicus
1.1.99.4 expressed in Gluconobacter japonicas strain NBRC3271 Gluconobacter oxydans

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.215 additional information a mutant strain of Gluconobacter japonicus NBRC3271 is engineered to produce 2-dehydro-D-gluconate efficiently from a mixture of D-glucose and D-gluconate Gluconobacter japonicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.215 membrane
-
 Gluconobacter japonicus 16020
-
1.1.99.4 membrane
-
 Gluconobacter oxydans 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.215 D-gluconate + NADP+ Gluconobacter japonicus
-
 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.215 D-gluconate + NADP+ Gluconobacter japonicus NBRC3271
-
 2-dehydro-D-gluconate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.215 Gluconobacter japonicus
-
-
-
1.1.1.215 Gluconobacter japonicus NBRC3271
-
-
-
1.1.99.4 Gluconobacter oxydans A0A0B6VQJ4 and A0A0B6VN56 and A0A0B6VKP6 cytochrome c subunit and large subunit and small subunit
-
1.1.99.4 Gluconobacter oxydans NBRC3293 A0A0B6VQJ4 and A0A0B6VN56 and A0A0B6VKP6 cytochrome c subunit and large subunit and small subunit
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.215 D-gluconate + NADP+
-
 Gluconobacter japonicus 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.1.215 D-gluconate + NADP+
-
 Gluconobacter japonicus NBRC3271 2-dehydro-D-gluconate + NADPH + H+
-
 ?
1.1.99.4 2-dehydro-D-gluconate + ferricyanide
-
 Gluconobacter oxydans 2,5-didehydro-D-gluconate + ferrocyanide
-
 ?
1.1.99.4 2-dehydro-D-gluconate + ferricyanide
-
 Gluconobacter oxydans NBRC3293 2,5-didehydro-D-gluconate + ferrocyanide
-
 ?
1.1.99.4 additional information the enzyme is completely inert toward 5-oxoglutarate, D-gluconate, D-glucose, D-sorbitol, L-lactate, L-malate, and D-fructose Gluconobacter oxydans ?
-
 ?
1.1.99.4 additional information the enzyme is completely inert toward 5-oxoglutarate, D-gluconate, D-glucose, D-sorbitol, L-lactate, L-malate, and D-fructose Gluconobacter oxydans NBRC3293 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.99.4 trimer a small subunit, a large subunit, and a cytochrome c subunit Gluconobacter oxydans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.215 2-ketogluconate dehydrogenase
-
 Gluconobacter japonicus
1.1.1.215 GADH
-
 Gluconobacter japonicus
1.1.99.4 2-keto-gluconate dehydrogenase
-
 Gluconobacter oxydans
1.1.99.4 2KGDH
-
 Gluconobacter oxydans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.215 NADP+
-
 Gluconobacter japonicus

General Information

EC Number General Information Comment Organism
1.1.1.215 physiological function Gluconobacter oxydans NBRC3293 produces 2,5-dioxo-D-gluconate from D-glucose via D-gluconate and 2-keto-D-gluconate, with accumulation of the product in the culture medium, the efficiency of 2,5-diketo-D-gluconate production is unsatisfactory because there is a large amount of residual D-gluconate at the end of the biotransformation process. Heterologous overexpression of the kgdSLC genes in a mutant strain of Gluconobacter japonicus NBRC3271 engineered to produce 2-dehydro-D-gluconate efficiently from a mixture of D-glucose and D-gluconate, results in a mutant strain that consumes almost all of the starting materials (D-glucose and D-gluconate) to produce 2,5-dioxo-D-gluconate quantitatively as a seemingly unique metabolite Gluconobacter japonicus