BRENDA - Enzyme Database show

Functional heterologous production of reductive dehalogenases from Desulfitobacterium hafniense strains

Mac Nelly, A.; Kai, M.; Svatos, A.; Diekert, G.; Schubert, T.; Appl. Environ. Microbiol. 80, 4313-4322 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.21.99.5
expression in Shimwellia blattae; expression in Shimwellia blattae
Desulfitobacterium hafniense
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.21.99.5
synthesis
the expression of catalytically active PceA enzyme in Shimwellia blattae is significantly enhanced when its dedicated PceT chaperone is coproduced and when 5,6-dimethylbenzimidazole and hydroxocobalamin are added to the cultures; the expression of catalytically active PceA enzyme in Shimwellia blattae is significantly enhanced when its dedicated PceT chaperone is coproduced and when 5,6-dimethylbenzimidazole and hydroxocobalamin are added to the cultures
Desulfitobacterium hafniense
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.21.99.5
Desulfitobacterium hafniense
B8FVX4
-
-
1.21.99.5
Desulfitobacterium hafniense
Q8L172
-
-
1.21.99.5
Desulfitobacterium hafniense DSM 10664
B8FVX4
-
-
1.21.99.5
Desulfitobacterium hafniense Y51
Q8L172
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.21.99.5
2,3-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
2-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,3-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense DSM 10664
2-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4,5-trichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
3,4-dichlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4,5-trichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense DSM 10664
3,4-dichlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4,6-trichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
4-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4,6-trichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense DSM 10664
4-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
4-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense DSM 10664
4-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
3,5-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
? + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
additional information
no substrates: 2,6-dichlorophenol, 3,4-dichlorophenol
739904
Desulfitobacterium hafniense
?
-
-
-
-
1.21.99.5
additional information
no substrates: 2,6-dichlorophenol, 3,4-dichlorophenol
739904
Desulfitobacterium hafniense DSM 10664
?
-
-
-
-
1.21.99.5
tetrachloroethene + reduced acceptor
-
739904
Desulfitobacterium hafniense
trichloroethene + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
tetrachloroethene + reduced acceptor
-
739904
Desulfitobacterium hafniense Y51
trichloroethene + chloride + oxidized acceptor
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.21.99.5
expression in Shimwellia blattae
Desulfitobacterium hafniense
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.21.99.5
synthesis
the expression of catalytically active PceA enzyme in Shimwellia blattae is significantly enhanced when its dedicated PceT chaperone is coproduced and when 5,6-dimethylbenzimidazole and hydroxocobalamin are added to the cultures
Desulfitobacterium hafniense
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.21.99.5
2,3-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
2-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,3-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense DSM 10664
2-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4,5-trichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
3,4-dichlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4,5-trichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense DSM 10664
3,4-dichlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4,6-trichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
4-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4,6-trichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense DSM 10664
4-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
4-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
2,4-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense DSM 10664
4-chlorophenol + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
3,5-dichlorophenol + reduced acceptor
-
739904
Desulfitobacterium hafniense
? + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
additional information
no substrates: 2,6-dichlorophenol, 3,4-dichlorophenol
739904
Desulfitobacterium hafniense
?
-
-
-
-
1.21.99.5
additional information
no substrates: 2,6-dichlorophenol, 3,4-dichlorophenol
739904
Desulfitobacterium hafniense DSM 10664
?
-
-
-
-
1.21.99.5
tetrachloroethene + reduced acceptor
-
739904
Desulfitobacterium hafniense
trichloroethene + chloride + oxidized acceptor
-
-
-
?
1.21.99.5
tetrachloroethene + reduced acceptor
-
739904
Desulfitobacterium hafniense Y51
trichloroethene + chloride + oxidized acceptor
-
-
-
?