EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.47 | expressed in Escherichia coli BL21(DE3) cells | Bacillus amyloliquefaciens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.47 | acetone | 1% residual activity at 50% (v/v) | Bacillus amyloliquefaciens | |
1.1.1.47 | ethanol | 19% residual activity at 50% (v/v) | Bacillus amyloliquefaciens | |
1.1.1.47 | n-butanol | 1% residual activity at 50% (v/v) | Bacillus amyloliquefaciens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 0.05 | - |
NADP+ | at pH 8.0 and 37°C | Bacillus amyloliquefaciens | |
1.1.1.47 | 0.25 | - |
NAD+ | at pH 8.0 and 37°C | Bacillus amyloliquefaciens | |
1.1.1.47 | 5.5 | - |
D-glucose | with NADP+ as cosubstrate,at pH 8.0 and 37°C | Bacillus amyloliquefaciens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 28000 | - |
- |
Bacillus amyloliquefaciens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.47 | D-glucose + NAD+ | Bacillus amyloliquefaciens | - |
D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | D-glucose + NAD+ | Bacillus amyloliquefaciens SB5 | - |
D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | D-glucose + NADP+ | Bacillus amyloliquefaciens | - |
D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
1.1.1.47 | D-glucose + NADP+ | Bacillus amyloliquefaciens SB5 | - |
D-glucono-1,5-lactone + NADPH + H+ | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
1.1.1.47 | 1-hexanol | the enzyme exhibits an extreme tolerance towards 1-hexanol with 6%loss of activity at 10% (v/v) | Bacillus amyloliquefaciens |
1.1.1.47 | 2-hexanol | the enzyme exhibits an extreme tolerance towards 2-hexanol with 7%loss of activity at 50% (v/v) | Bacillus amyloliquefaciens |
1.1.1.47 | DMSO | the enzyme exhibits an extreme tolerance towards DMSO with 1% loss of activity at 50% (v/v) | Bacillus amyloliquefaciens |
1.1.1.47 | n-hexane | the enzyme exhibits an extreme tolerance towards n-hexane with no loss of activity at 50% (v/v) | Bacillus amyloliquefaciens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.47 | Bacillus amyloliquefaciens | - |
- |
- |
1.1.1.47 | Bacillus amyloliquefaciens SB5 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.47 | PrepEase histidine-tagged protein purification kits | Bacillus amyloliquefaciens |
EC Number | Storage Stability | Organism |
---|---|---|
1.1.1.47 | -80°C, in CPB buffer (pH 6.0), at least 4 months, no loss of activity | Bacillus amyloliquefaciens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.47 | D-glucose + NAD+ | - |
Bacillus amyloliquefaciens | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | D-glucose + NAD+ | - |
Bacillus amyloliquefaciens SB5 | D-glucono-1,5-lactone + NADH + H+ | - |
? | |
1.1.1.47 | D-glucose + NADP+ | - |
Bacillus amyloliquefaciens | D-glucono-1,5-lactone + NADPH + H+ | - |
? | |
1.1.1.47 | D-glucose + NADP+ | - |
Bacillus amyloliquefaciens SB5 | D-glucono-1,5-lactone + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.47 | ? | x * 28000, His6-tagged enzyme, SDS-PAGE | Bacillus amyloliquefaciens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.47 | glucose dehydrogenase | - |
Bacillus amyloliquefaciens |
1.1.1.47 | GluDH | - |
Bacillus amyloliquefaciens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 50 | - |
- |
Bacillus amyloliquefaciens |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 42 | - |
the enzyme is stable up to 42°C | Bacillus amyloliquefaciens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.47 | 70 | - |
D-glucose | at pH 8.0 and 37°C | Bacillus amyloliquefaciens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 10 | - |
- |
Bacillus amyloliquefaciens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.47 | 5 | 8 | when incubated for 6 h at 37°C, the enzyme is highly stable in acidic conditions (pH 5.0-6.0) but is completely inactivated at pH values above 8.0 | Bacillus amyloliquefaciens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.47 | NAD+ | - |
Bacillus amyloliquefaciens | |
1.1.1.47 | NADP+ | - |
Bacillus amyloliquefaciens |